Opened 3 weeks ago
Last modified 3 weeks ago
#20424 assigned defect
glBlitFramebuffer: invalid framebuffer operation after Unsupported scale factor detected
| Reported by: | Owned by: | Tom Goddard | |
|---|---|---|---|
| Priority: | normal | Milestone: | |
| Component: | Graphics | Version: | |
| Keywords: | Cc: | ||
| Blocked By: | Blocking: | ||
| Notify when closed: | Platform: | all | |
| Project: | ChimeraX |
Description
The following bug report has been submitted:
Platform: macOS-26.5-arm64-arm-64bit
ChimeraX Version: 1.11.1 (2026-01-23 05:51:34 UTC)
Description
Replace this text with list of actions that caused this problem to occur
Log:
UCSF ChimeraX version: 1.11.1 (2026-01-23)
© 2016-2025 Regents of the University of California. All rights reserved.
How to cite UCSF ChimeraX
> open /Users/tomforan/Library/CloudStorage/OneDrive-
> Birkbeck,UniversityofLondon/modelling/2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb
> format pdb
Chain information for 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb #1
---
Chain | Description
A B | No description available
C F H a | No description available
D | No description available
E G M b | No description available
> cd
> /Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J290_map_sharp_all_classes_sharp/
Current working directory is:
/Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J290_map_sharp_all_classes_sharp
> open *.mrc vseries true
Opened map series J290_volume_class_00_map_sharp.mrc as #2, 20 images, grid
size 180,180,180, pixel 1.66, shown at level 0.234, step 1, values float32
> ui tool show "Fit in Map"
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J290_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.07496, steps = 180
shifted from previous position = 37.8
rotated from previous position = 0.183 degrees
atoms outside contour = 64361, contour level = 0.23397
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J290_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.99999528 0.00241246 -0.00190198 29.01398635
-0.00241409 0.99999672 -0.00085453 -23.60032147
0.00189991 0.00085912 0.99999783 2.63445825
Axis 0.26865575 -0.59603790 -0.75667887
Axis point -4857.31806057 -9823.67011246 0.00000000
Rotation angle (degrees) 0.18273391
Shift along axis 19.86802144
> ui mousemode right "translate selected models"
> select add #2
22 models selected
> view matrix models #2,1,0,0,5.9383,0,1,0,10.907,0,0,1,17.491
> undo
> select add #1
69260 atoms, 70003 bonds, 28 pseudobonds, 4460 residues, 25 models selected
> select subtract #2
69260 atoms, 70003 bonds, 28 pseudobonds, 4460 residues, 3 models selected
> view matrix models
> #1,1,0.0024125,-0.001902,58.232,-0.0024141,1,-0.00085453,20.362,0.0018999,0.00085912,1,43.838
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J290_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.1103, steps = 164
shifted from previous position = 24.3
rotated from previous position = 27.7 degrees
atoms outside contour = 57262, contour level = 0.23397
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J290_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.88456862 0.46640640 -0.00185352 0.69049043
-0.46640664 0.88457037 0.00032648 85.29480865
0.00179184 0.00057570 0.99999823 52.74037267
Axis 0.00026717 -0.00390790 -0.99999233
Axis point 172.25495990 41.12070230 0.00000000
Rotation angle (degrees) 27.80151932
Shift along axis -53.07310700
> volume #2.1 level 0.1406
> select clear
> volume #2.1 level 0.07543
> volume #2.2 color #b2b2b299
Drag select of 2.5 J290_volume_class_04_map_sharp.mrc , 22 atoms
> select clear
> select /D:236@CG2
1 atom, 1 residue, 1 model selected
> select add /A:73@CG
2 atoms, 2 residues, 1 model selected
> select add /B:64@OD1
3 atoms, 3 residues, 1 model selected
> select up
43 atoms, 40 bonds, 3 residues, 1 model selected
> select up
242 atoms, 242 bonds, 14 residues, 1 model selected
> select up
15561 atoms, 15684 bonds, 992 residues, 1 model selected
> molmap sel 12 onGrid #2.4
Opened 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb map 12 as #3, grid size
180,180,180, pixel 1.66, shown at level 0.0483, step 1, values float32
> save "/Users/tomforan/OneDrive - Birkbeck, University of
> London/references/2DC+linker_2Kif_P501_J290.mrc" models #3
> close #2
> cd
> /Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J298_map_sharp_all_classes_sharp/
Current working directory is:
/Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J298_map_sharp_all_classes_sharp
> open *.mrc vseries true
Opened map series J298_volume_class_00_map_sharp.mrc as #2, 20 images, grid
size 180,180,180, pixel 1.66, shown at level 0.243, step 1, values float32
> volume #2.1 level 0.118
> hide #!3 models
> color #2 #b2ffff98 models
> color #2 #f4fef697 models
> select clear
> hide atoms
> show cartoons
> ui tool show "Hide Dust"
> volume #2.6 level 0.09208
> volume #2.6 level 0.1163
> select add #1
69260 atoms, 70003 bonds, 28 pseudobonds, 4460 residues, 3 models selected
> view sel
> select clear
> select add #1
69260 atoms, 70003 bonds, 28 pseudobonds, 4460 residues, 3 models selected
> ui tool show "Surface Zone"
> surface zone #3 nearAtoms #1 distance 9.94
> surface zone #2.1 nearAtoms #1 distance 9.94
> close #2
> open *.mrc vseries true
Opened map series J298_volume_class_00_map_sharp.mrc as #2, 20 images, grid
size 180,180,180, pixel 1.66, shown at level 0.243, step 1, values float32
> volume #2.1 level 0.1591
> surface dust #3 size 9.94
> volume #2.5 level 0.103
> volume #2.5 level 0.1505
> volume #2.7 level 0.1521
> volume #2.9 level 0.1381
> close #2
> cd
> /Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J302_map_sharp_all_classes_sharp/
Current working directory is:
/Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J302_map_sharp_all_classes_sharp
> open *.mrc vseries true
Opened map series J302_volume_class_00_map_sharp.mrc as #2, 5 images, grid
size 180,180,180, pixel 3.31, shown at level 0.669, step 1, values float32
> volume #2.1 level 0.2329
> ui tool show "Side View"
> volume #2.1 level 0.1015
> volume #2.1 level 0.028
> view matrix models
> #1,0.88457,0.46641,-0.0018535,25.235,-0.46641,0.88457,0.00032648,234.78,0.0017918,0.0005757,1,60.148
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J302_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.4703, steps = 100
shifted from previous position = 34.8
rotated from previous position = 28.7 degrees
atoms outside contour = 4788, contour level = 0.028
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J302_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.99984956 -0.01629828 0.00593447 94.71027491
0.01631940 0.99986061 -0.00352660 183.92275185
-0.00587617 0.00362292 0.99997617 76.39445162
Axis 0.20185451 0.33345311 0.92090378
Axis point -8154.59793709 4018.67593995 0.00000000
Rotation angle (degrees) 1.01473853
Shift along axis 150.79924962
> view matrix models
> #1,0.99985,-0.016298,0.0059345,97.813,0.016319,0.99986,-0.0035266,183.11,-0.0058762,0.0036229,0.99998,191.89
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J302_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.4918, steps = 68
shifted from previous position = 7.33
rotated from previous position = 0.157 degrees
atoms outside contour = 4887, contour level = 0.028
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J302_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.99988384 -0.01368349 0.00671388 94.31097644
0.01370842 0.99989926 -0.00368107 184.29222468
-0.00666283 0.00377268 0.99997069 198.50466162
Axis 0.23751872 0.42625842 0.87286231
Axis point -4900.85384983 2173.93054781 0.00000000
Rotation angle (degrees) 0.89905639
Shift along axis 274.22397323
> view matrix models
> #1,0.99988,-0.013683,0.0067139,91.714,0.013708,0.9999,-0.0036811,185.69,-0.0066628,0.0037727,0.99997,154.63
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J302_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.4892, steps = 56
shifted from previous position = 4.29
rotated from previous position = 0.0539 degrees
atoms outside contour = 5033, contour level = 0.028
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J302_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.99987635 -0.01445787 0.00618471 94.51907263
0.01448029 0.99988869 -0.00359590 184.20426506
-0.00613203 0.00368501 0.99997441 157.63561467
Axis 0.22554027 0.38153536 0.89641640
Axis point -6561.54497111 2960.38321540 0.00000000
Rotation angle (degrees) 0.92485284
Shift along axis 232.90544699
> volume #2.2 color #ffb2ff96
> volume #2.2 level 0.6847
> view matrix models
> #1,0.99988,-0.014458,0.0061847,265.48,0.01448,0.99989,-0.0035959,83.735,-0.006132,0.003685,0.99997,147.06
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J302_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.4196, steps = 160
shifted from previous position = 59.7
rotated from previous position = 43.1 degrees
atoms outside contour = 7901, contour level = 0.028
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J302_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.74072230 0.67180540 0.00282463 251.32460407
-0.67120066 0.74022167 -0.03951656 225.35593865
-0.02863829 0.02737491 0.99921492 154.57630564
Axis 0.04973199 0.02339182 -0.99848863
Axis point 425.63573832 -219.94280029 0.00000000
Rotation angle (degrees) 42.26181666
Shift along axis -136.57232370
> view matrix models
> #1,0.74072,0.67181,0.0028246,189.95,-0.6712,0.74022,-0.039517,171.75,-0.028638,0.027375,0.99921,242.39
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J302_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.385, steps = 128
shifted from previous position = 31.3
rotated from previous position = 31.7 degrees
atoms outside contour = 9696, contour level = 0.028
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J302_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.27773698 0.96061461 -0.00904073 227.10943623
-0.96036349 0.27740710 -0.02733614 264.85484598
-0.02375154 0.01627464 0.99958541 246.19725283
Axis 0.02269587 0.00765578 -0.99971310
Axis point 293.68241604 -21.25362389 0.00000000
Rotation angle (degrees) 73.89701255
Shift along axis -238.94450183
> view matrix models
> #1,0.27774,0.96061,-0.0090407,173.97,-0.96036,0.27741,-0.027336,244.8,-0.023752,0.016275,0.99959,244.98
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J302_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.3401, steps = 116
shifted from previous position = 12.4
rotated from previous position = 27.2 degrees
atoms outside contour = 10853, contour level = 0.028
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J302_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
-0.19243661 0.98099577 -0.02480808 216.93907723
-0.98093425 -0.19300055 -0.02277705 302.81973578
-0.02713217 0.01995196 0.99943272 254.60356211
Axis 0.02177389 0.00118431 -0.99976222
Axis point 235.92197056 60.09321156 0.00000000
Rotation angle (degrees) 101.12804242
Shift along axis -249.46078199
> ui mousemode right "rotate selected models"
> view matrix models
> #1,0.094606,-0.99225,-0.080497,405.57,0.97502,0.10868,-0.19372,125.22,0.20097,-0.060159,0.97775,246.16
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J302_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.3996, steps = 88
shifted from previous position = 26.2
rotated from previous position = 12.4 degrees
atoms outside contour = 7173, contour level = 0.028
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J302_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.10866699 -0.99407400 0.00290134 386.96841389
0.99406704 0.10867916 0.00442812 81.33142087
-0.00471719 0.00240294 0.99998601 251.74439322
Axis -0.00101862 0.00383195 0.99999214
Axis point 148.79656542 256.11521752 0.00000000
Rotation angle (degrees) 83.76157346
Shift along axis 251.65989762
> ui mousemode right "translate selected models"
> view matrix models
> #1,0.10867,-0.99407,0.0029013,392.41,0.99407,0.10868,0.0044281,78.146,-0.0047172,0.0024029,0.99999,218.78
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J302_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.4209, steps = 72
shifted from previous position = 10.2
rotated from previous position = 0.115 degrees
atoms outside contour = 3995, contour level = 0.028
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J302_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.10701149 -0.99425341 0.00295689 387.12767570
0.99425120 0.10702116 0.00332575 81.67335672
-0.00362308 0.00258400 0.99999012 210.72539971
Axis -0.00037302 0.00330899 0.99999446
Axis point 148.52354887 256.04791513 0.00000000
Rotation angle (degrees) 83.85693665
Shift along axis 210.85008218
> view sel
> volume #2.2 level 0.03942
Unsupported scale factor (0.000000) detected on Display0
[Repeated 3 time(s)]
> open "/Users/tomforan/Library/CloudStorage/OneDrive-
> Birkbeck,UniversityofLondon/Temporary
> Maps/cryosparc_P501_J321_class_00_01200_volume.mrc"
> "/Users/tomforan/Library/CloudStorage/OneDrive-
> Birkbeck,UniversityofLondon/Temporary
> Maps/cryosparc_P501_J321_class_01_01200_volume.mrc"
Opened cryosparc_P501_J321_class_00_01200_volume.mrc as #4.1, grid size
128,128,128, pixel 1.66, shown at level 0.0828, step 1, values float32
Opened cryosparc_P501_J321_class_01_01200_volume.mrc as #4.2, grid size
128,128,128, pixel 1.66, shown at level 0.134, step 1, values float32
> open 6zpd
Summary of feedback from opening 6zpd fetched from pdb
---
notes | Fetching compressed mmCIF 6zpd from http://files.rcsb.org/download/6zpd.cif
Fetching CCD CL from
https://files.wwpdb.org/pub/pdb/refdata/chem_comp/L/CL/CL.cif
Fetching CCD SO4 from
https://files.wwpdb.org/pub/pdb/refdata/chem_comp/4/SO4/SO4.cif
Fetching CCD TRS from
https://files.wwpdb.org/pub/pdb/refdata/chem_comp/S/TRS/TRS.cif
Fetching CCD VIV from
https://files.wwpdb.org/pub/pdb/refdata/chem_comp/V/VIV/VIV.cif
6zpd title:
gamma-tocopherol transfer protein [more info...]
Chain information for 6zpd #5
---
Chain | Description | UniProt
A | Alpha-tocopherol transfer protein | TTPA_HUMAN 48-278
Non-standard residues in 6zpd #5
---
CL — chloride ion
SO4 — sulfate ion
TRS — 2-amino-2-hydroxymethyl-propane-1,3-diol (tris buffer)
VIV —
(2R)-2,5,7,8-tetramethyl-2-[(4R,8R)-4,8,12-trimethyltridecyl]chroman-6-ol
6zpd mmCIF Assemblies
---
1| author_and_software_defined_assembly
29 atoms have alternate locations. Control/examine alternate locations with
Altloc Explorer [start tool...] or the altlocs command.
1904 atoms have anisotropic B-factors. Depict anisotropic information with
Thermal Ellipsoids [start tool...] or the aniso command.
> open 6zph
Summary of feedback from opening 6zph fetched from pdb
---
notes | Fetching compressed mmCIF 6zph from http://files.rcsb.org/download/6zph.cif
Fetching CCD ADP from
https://files.wwpdb.org/pub/pdb/refdata/chem_comp/P/ADP/ADP.cif
6zph title:
Kinesin binding protein complexed with Kif15 motor domain [more info...]
Chain information for 6zph #6
---
Chain | Description | UniProt
A | KIF-binding protein | KBP_HUMAN 1-621
B | Kinesin-like protein KIF15 | KIF15_HUMAN 1-374
Non-standard residues in 6zph #6
---
ADP — adenosine-5'-diphosphate
MG — magnesium ion
> fitmap #6 inMap #4.1
Fit molecule 6zph (#6) to map cryosparc_P501_J321_class_00_01200_volume.mrc
(#4.1) using 6226 atoms
average map value = 0.1135, steps = 180
shifted from previous position = 14.8
rotated from previous position = 35.3 degrees
atoms outside contour = 3766, contour level = 0.082781
Position of 6zph (#6) relative to
cryosparc_P501_J321_class_00_01200_volume.mrc (#4.1) coordinates:
Matrix rotation and translation
0.81650764 -0.25769753 0.51663068 -0.45898497
0.26401238 0.96247091 0.06282681 -12.49460498
-0.51343231 0.08509833 0.85390019 55.66743832
Axis 0.01928500 0.89193574 0.45175074
Axis point 86.92248820 -0.00000000 32.28318332
Rotation angle (degrees) 35.27006673
Shift along axis 13.99457020
> volume #4.1 level 0.1626
> fitmap #6 inMap #4.1
Fit molecule 6zph (#6) to map cryosparc_P501_J321_class_00_01200_volume.mrc
(#4.1) using 6226 atoms
average map value = 0.1135, steps = 108
shifted from previous position = 0.00982
rotated from previous position = 0.0126 degrees
atoms outside contour = 4800, contour level = 0.16265
Position of 6zph (#6) relative to
cryosparc_P501_J321_class_00_01200_volume.mrc (#4.1) coordinates:
Matrix rotation and translation
0.81638649 -0.25779981 0.51677109 -0.44211092
0.26414323 0.96243433 0.06283717 -12.50184082
-0.51355765 0.08520217 0.85381446 55.68126795
Axis 0.01936017 0.89190006 0.45181796
Axis point 86.92257846 0.00000000 32.26436466
Rotation angle (degrees) 35.28214350
Shift along axis 13.99884513
> fitmap #6 inMap #4.1
Fit molecule 6zph (#6) to map cryosparc_P501_J321_class_00_01200_volume.mrc
(#4.1) using 6226 atoms
average map value = 0.1135, steps = 76
shifted from previous position = 0.0174
rotated from previous position = 0.0265 degrees
atoms outside contour = 4800, contour level = 0.16265
Position of 6zph (#6) relative to
cryosparc_P501_J321_class_00_01200_volume.mrc (#4.1) coordinates:
Matrix rotation and translation
0.81634997 -0.25808398 0.51668693 -0.41672214
0.26419724 0.96238997 0.06328789 -12.55346775
-0.51358791 0.08484220 0.85383210 55.71242869
Axis 0.01865696 0.89178442 0.45207573
Axis point 86.99475979 0.00000000 32.23267384
Rotation angle (degrees) 35.28527964
Shift along axis 13.98347512
> fitmap #6 inMap #4.2
Fit molecule 6zph (#6) to map cryosparc_P501_J321_class_01_01200_volume.mrc
(#4.2) using 6226 atoms
average map value = 0.1754, steps = 212
shifted from previous position = 24.2
rotated from previous position = 46 degrees
atoms outside contour = 3205, contour level = 0.13385
Position of 6zph (#6) relative to
cryosparc_P501_J321_class_01_01200_volume.mrc (#4.2) coordinates:
Matrix rotation and translation
0.52825370 0.40631348 0.74555844 -43.89436843
-0.35253549 0.90376518 -0.24274931 69.43068757
-0.77244207 -0.13460259 0.62065722 139.15677629
Axis 0.06359511 0.89265226 -0.44623717
Axis point 114.19458209 0.00000000 130.88191857
Rotation angle (degrees) 58.24163592
Shift along axis -2.91093296
> volume #4.2 color #b2ffb29c
> volume #4.2 level 0.3379
> select add #6
75486 atoms, 76336 bonds, 43 pseudobonds, 5234 residues, 7 models selected
> ui mousemode right "rotate selected models"
> view matrix models
> #1,0.44128,-0.88245,0.16296,347.9,0.8934,0.41498,-0.17211,21.465,0.08425,0.22154,0.97151,162.32,#6,0.24423,0.66612,0.70473,-66.442,-0.36171,0.73686,-0.57114,159.37,-0.89973,-0.11542,0.4209,181.23
> view matrix models
> #1,0.054081,-0.99538,-0.07932,390.75,0.95717,0.074303,-0.27982,84.491,0.28442,-0.06079,0.95677,206.42,#6,0.60782,0.3675,0.70391,-31.68,-0.10465,0.9158,-0.38776,81.065,-0.78714,0.16202,0.59511,94.218
> view matrix models
> #1,-0.24114,-0.78358,0.57258,359.06,0.57837,-0.5898,-0.56357,237.19,0.77932,0.19526,0.59543,135.79,#6,0.070838,-0.060911,0.99563,-3.3073,0.5999,0.80005,0.0062637,-7.1798,-0.79693,0.59683,0.093215,130.55
> fitmap #6 inMap #4.2
Fit molecule 6zph (#6) to map cryosparc_P501_J321_class_01_01200_volume.mrc
(#4.2) using 6226 atoms
average map value = 0.1754, steps = 364
shifted from previous position = 36.3
rotated from previous position = 64.6 degrees
atoms outside contour = 5074, contour level = 0.3379
Position of 6zph (#6) relative to
cryosparc_P501_J321_class_01_01200_volume.mrc (#4.2) coordinates:
Matrix rotation and translation
0.52837324 0.40635795 0.74544949 -43.89848246
-0.35250566 0.90376267 -0.24280194 69.43182850
-0.77237392 -0.13448512 0.62076749 139.12452985
Axis 0.06370042 0.89262198 -0.44628271
Axis point 114.18317344 0.00000000 130.88080350
Rotation angle (degrees) 58.23397757
Shift along axis -2.90884665
> view matrix models
> #1,-0.19425,-0.77314,0.60375,354.71,0.56155,-0.59229,-0.57779,238.84,0.80431,0.2268,0.54922,130.21,#6,0.48218,0.40108,0.77887,-47.963,-0.33651,0.90564,-0.25803,71.916,-0.80887,-0.13768,0.57164,152.92
> view sel
> select clear
> select add #6
6226 atoms, 6333 bonds, 15 pseudobonds, 774 residues, 4 models selected
> view sel
> view matrix models
> #6,-0.40631,0.39651,-0.82322,172.38,0.26245,0.91362,0.31052,-33.339,0.87523,-0.089885,-0.47528,80.832
> fitmap #6 inMap #4.2
Fit molecule 6zph (#6) to map cryosparc_P501_J321_class_01_01200_volume.mrc
(#4.2) using 6226 atoms
average map value = 0.1756, steps = 144
shifted from previous position = 12.2
rotated from previous position = 27.7 degrees
atoms outside contour = 5108, contour level = 0.3379
Position of 6zph (#6) relative to
cryosparc_P501_J321_class_01_01200_volume.mrc (#4.2) coordinates:
Matrix rotation and translation
-0.71772120 0.44010120 -0.53961766 182.05107172
0.42507190 0.89071202 0.16107760 -35.75629507
0.55153437 -0.11376749 -0.82635755 149.92412401
Axis -0.24423406 -0.96962434 -0.01335541
Axis point 71.74011954 0.00000000 103.66739907
Rotation angle (degrees) 145.75953151
Shift along axis -11.79519631
> fitmap #4.1 inMap #4.2
Fit map cryosparc_P501_J321_class_00_01200_volume.mrc in map
cryosparc_P501_J321_class_01_01200_volume.mrc using 9665 points
correlation = 0.8435, correlation about mean = 0.3112, overlap = 924.1
steps = 156, shift = 2.46, angle = 44.3 degrees
Position of cryosparc_P501_J321_class_00_01200_volume.mrc (#4.1) relative to
cryosparc_P501_J321_class_01_01200_volume.mrc (#4.2) coordinates:
Matrix rotation and translation
0.76471150 -0.43264984 -0.47752533 121.65703621
0.56617173 0.80498612 0.17733282 -57.39908676
0.30767825 -0.40596979 0.86053624 22.76978220
Axis -0.41723789 -0.56165818 0.71445968
Axis point 168.21254292 187.53838610 0.00000000
Rotation angle (degrees) 44.34721729
Shift along axis -2.25316723
> fitmap #6 inMap #4.1
Fit molecule 6zph (#6) to map cryosparc_P501_J321_class_00_01200_volume.mrc
(#4.1) using 6226 atoms
average map value = 0.1274, steps = 340
shifted from previous position = 17.9
rotated from previous position = 54.8 degrees
atoms outside contour = 4432, contour level = 0.16265
Position of 6zph (#6) relative to
cryosparc_P501_J321_class_00_01200_volume.mrc (#4.1) coordinates:
Matrix rotation and translation
-0.47526998 0.84561550 -0.24300798 86.39833544
-0.24151031 0.14020285 0.96021661 17.36256056
0.84604446 0.51505106 0.13759066 -41.51085362
Axis -0.27790028 -0.67985489 -0.67865217
Axis point 56.85631841 0.00000000 1.62959264
Rotation angle (degrees) 126.77958382
Shift along axis -7.64271263
> volume #4.1 color #ffb2b2a3
Unsupported scale factor (0.000000) detected on Display0
[Repeated 1 time(s)]
> close #6
> close #2
> close #5
> show #!4.2 models
> close #4.2
> close #4.1
> cd
> /Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J327_map_sharp_all_classes_sharp/
Current working directory is:
/Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J327_map_sharp_all_classes_sharp
> open *.mrc vseries true
Opened map series J327_volume_class_00_map_sharp.mrc as #2, 10 images, grid
size 90,90,90, pixel 6.62, shown at level 1.87, step 1, values float32
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J327_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.9553, steps = 148
shifted from previous position = 36.7
rotated from previous position = 63.2 degrees
atoms outside contour = 64807, contour level = 1.8717
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J327_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.07961603 -0.74136797 0.66635941 308.00710149
0.99132877 0.12899178 0.02506863 81.95834690
-0.10453995 0.65858538 0.74520919 181.90298488
Axis 0.31684286 0.38555248 0.86657942
Axis point 136.88944302 122.93301850 0.00000000
Rotation angle (degrees) 91.32316327
Shift along axis 286.82247669
> select add #1
69260 atoms, 70003 bonds, 28 pseudobonds, 4460 residues, 3 models selected
> ui mousemode right "rotate selected models"
> view matrix models
> #1,0.12946,-0.99051,0.046171,386.1,0.98845,0.12522,-0.085323,92.566,0.078732,0.056684,0.99528,207.42
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J327_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 1.164, steps = 56
shifted from previous position = 11.8
rotated from previous position = 5.95 degrees
atoms outside contour = 62446, contour level = 1.8717
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J327_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.10204025 -0.99471311 0.01156132 387.67674262
0.99472766 0.10214752 0.00910000 81.11397462
-0.01023284 0.01057180 0.99989178 209.99845036
Axis 0.00073976 0.01095426 0.99993973
Axis point 150.10938737 254.07160128 0.00000000
Rotation angle (degrees) 84.14335854
Shift along axis 211.16112598
> volume #2.1 level 0.4216
> close #2
> open "/Users/tomforan/Library/CloudStorage/OneDrive-
> Birkbeck,UniversityofLondon/Temporary
> Maps/cryosparc_P501_J245__localfilter.mrc"
Opened cryosparc_P501_J245__localfilter.mrc as #2, grid size 240,240,240,
pixel 0.828, shown at level 0.0988, step 1, values float32
> ui mousemode right "translate selected models"
> view matrix models
> #1,0.10204,-0.99471,0.011561,194.43,0.99473,0.10215,0.0091,-11.521,-0.010233,0.010572,0.99989,32.687
> fitmap #1 inMap #2
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
cryosparc_P501_J245__localfilter.mrc (#2) using 69260 atoms
average map value = 0.02272, steps = 664
shifted from previous position = 46.9
rotated from previous position = 40.3 degrees
atoms outside contour = 65301, contour level = 0.098768
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
cryosparc_P501_J245__localfilter.mrc (#2) coordinates:
Matrix rotation and translation
0.71740167 -0.69653141 -0.01337458 118.72587124
0.69372971 0.71601161 -0.07788791 -64.96193125
0.06382774 0.04659857 0.99687243 -16.01986152
Axis 0.08904888 -0.05522512 0.99449509
Axis point 140.34336266 113.73908254 0.00000000
Rotation angle (degrees) 44.34509434
Shift along axis -1.77173799
> view sel
> view matrix models
> #1,0.7174,-0.69653,-0.013375,119.64,0.69373,0.71601,-0.077888,-24.276,0.063828,0.046599,0.99687,-13.117
> fitmap #1 inMap #2
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
cryosparc_P501_J245__localfilter.mrc (#2) using 69260 atoms
average map value = 0.05265, steps = 448
shifted from previous position = 20.7
rotated from previous position = 44.3 degrees
atoms outside contour = 58430, contour level = 0.098768
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
cryosparc_P501_J245__localfilter.mrc (#2) coordinates:
Matrix rotation and translation
0.99999758 -0.00170744 0.00140206 0.05580501
0.00170665 0.99999840 0.00057704 -0.19947271
-0.00140304 -0.00057464 0.99999887 0.12139369
Axis -0.25221399 0.61430684 0.74767319
Axis point 100.40124062 25.76715776 0.00000000
Rotation angle (degrees) 0.13081459
Shift along axis -0.04584945
> fitmap #1 inMap #2
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
cryosparc_P501_J245__localfilter.mrc (#2) using 69260 atoms
average map value = 0.05265, steps = 44
shifted from previous position = 0.00204
rotated from previous position = 0.00152 degrees
atoms outside contour = 58433, contour level = 0.098768
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
cryosparc_P501_J245__localfilter.mrc (#2) coordinates:
Matrix rotation and translation
0.99999762 -0.00170538 0.00137751 0.05628982
0.00170458 0.99999840 0.00058671 -0.19933639
-0.00137850 -0.00058436 0.99999890 0.11917325
Axis -0.25805113 0.60729831 0.75139761
Axis point 100.56525584 25.89952372 0.00000000
Rotation angle (degrees) 0.13000859
Shift along axis -0.04603581
> view sel
> volume #2 color #99bfe596
> select clear
> volume #2 level 0.05451
> volume #2 level 0.04197
> lighting simple
> close #2
> cd
> /Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J306_map_sharp_all_classes_sharp/
Current working directory is:
/Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J306_map_sharp_all_classes_sharp
> open *.mrc vseries true
Opened map series J306_volume_class_00_map_sharp.mrc as #2, 6 images, grid
size 90,90,90, pixel 3.31, shown at level 1.09, step 1, values float32
> select add #1
69260 atoms, 70003 bonds, 28 pseudobonds, 4460 residues, 3 models selected
> view matrix models
> #1,1,-0.0017054,0.0013775,32.47,0.0017046,1,0.00058671,33.539,-0.0013785,-0.00058436,1,-1.5586
> fitmap #1 inMap #3
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
2DC+linker_2Kif_P501_J290.mrc (#3) using 69260 atoms
average map value = 0.06753, steps = 396
shifted from previous position = 61.8
rotated from previous position = 50.3 degrees
atoms outside contour = 49739, contour level = 0.048308
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
2DC+linker_2Kif_P501_J290.mrc (#3) coordinates:
Matrix rotation and translation
0.88705537 0.41392843 0.20444088 -36.38172945
-0.44329956 0.64004555 0.62755655 66.42663515
0.12891202 -0.64730595 0.75125012 134.57732436
Axis -0.82884363 0.04910460 -0.55732125
Axis point 0.00000000 175.37058929 -8.51442482
Rotation angle (degrees) 50.26963376
Shift along axis -41.58618411
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J306_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.2042, steps = 112
shifted from previous position = 33.1
rotated from previous position = 8.99 degrees
atoms outside contour = 64890, contour level = 1.0878
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J306_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.85473341 0.38970244 0.34287441 -12.70609069
-0.51561910 0.56143623 0.64724520 68.11214428
0.05973092 -0.73001467 0.68081630 153.43700592
Axis -0.82356713 0.16931276 -0.54135975
Axis point 0.00000000 168.09356882 -7.31097396
Rotation angle (degrees) 56.73631543
Shift along axis -61.06804502
> view matrix models
> #1,0.85473,0.3897,0.34287,13.584,-0.51562,0.56144,0.64725,84.745,0.059731,-0.73001,0.68082,152.72
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J306_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.2462, steps = 140
shifted from previous position = 21.9
rotated from previous position = 19.1 degrees
atoms outside contour = 64042, contour level = 1.0878
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J306_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.65356477 0.53668896 0.53368352 0.47084291
-0.74464110 0.32970406 0.58034894 157.21699546
0.13550924 -0.77669830 0.61512360 161.02961551
Axis -0.71109785 0.20864482 -0.67142177
Axis point 0.00000000 184.21403900 -85.33363161
Rotation angle (degrees) 72.59066804
Shift along axis -75.65109321
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J306_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.2462, steps = 44
shifted from previous position = 0.0117
rotated from previous position = 0.0222 degrees
atoms outside contour = 64031, contour level = 1.0878
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J306_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.65370894 0.53643753 0.53375974 0.48119741
-0.74451235 0.32959131 0.58057812 157.20533375
0.13552131 -0.77691981 0.61484113 161.08277509
Axis -0.71130468 0.20866983 -0.67119487
Axis point 0.00000000 184.22850046 -85.23248134
Rotation angle (degrees) 72.59820526
Shift along axis -75.65620022
> ui mousemode right "rotate selected models"
> view matrix models
> #1,0.72624,0.68535,-0.053539,31.92,-0.68741,0.72335,-0.065011,169.46,-0.0058272,0.084017,0.99645,47.826
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J306_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.4826, steps = 68
shifted from previous position = 7.55
rotated from previous position = 12.1 degrees
atoms outside contour = 58630, contour level = 1.0878
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J306_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.58021614 0.81446255 -0.00019100 25.36089842
-0.81446255 0.58021607 -0.00031870 191.37489425
-0.00014874 0.00034048 0.99999995 62.90679731
Axis 0.00040467 -0.00002594 -0.99999992
Axis point 198.34389269 71.05939500 0.00000000
Rotation angle (degrees) 54.53425934
Shift along axis -62.90149451
> volume #2.1 level 0.5999
> volume #2.1 color #d1d3d5
> volume #2.1 color #d1d3d590
> select clear
> volume #2.1 level 0.2857
> volume #2.2 level 0.1505
> volume #2.4 level 0.05759
> volume #2.4 level 0.6151
> volume #2.4 level 0.2434
> volume #2.4 level 0.6538
> volume #2.4 level 0.3828
> volume #2.3 level 0.25
Unsupported scale factor (0.000000) detected on Display0
[Repeated 5 time(s)]
> close #2
> cd
> /Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J341_map_sharp_all_classes_sharp/
Current working directory is:
/Users/tomforan/Desktop/temporary_maps_desktop/cryosparc_P501_J341_map_sharp_all_classes_sharp
> open *.mrc vseries true
Opened map series J341_volume_class_00_map_sharp.mrc as #2, 6 images, grid
size 90,90,90, pixel 3.31, shown at level 1.02, step 1, values float32
> volume #2.1 level 0.4555
> select add #1
69260 atoms, 70003 bonds, 28 pseudobonds, 4460 residues, 3 models selected
> view matrix models
> #1,0.59035,0.80715,0.00017213,25.261,-0.80714,0.59034,-0.00489,190.12,-0.0040486,0.0027479,0.99999,62.977
> ui mousemode right "translate selected models"
> view matrix models
> #1,0.59035,0.80715,0.00017213,27.576,-0.80714,0.59034,-0.00489,183.27,-0.0040486,0.0027479,0.99999,15.322
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J341_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.4061, steps = 72
shifted from previous position = 9.76
rotated from previous position = 0.679 degrees
atoms outside contour = 41180, contour level = 0.45548
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J341_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.58169274 0.81340848 -0.00050918 25.43390972
-0.81340850 0.58169288 0.00019860 190.75964953
0.00045773 0.00029864 0.99999987 22.06891975
Axis 0.00006150 -0.00059436 -0.99999982
Axis point 198.17322565 70.64350939 0.00000000
Rotation angle (degrees) 54.43031173
Shift along axis -22.18073071
> view matrix models
> #1,0.58169,0.81341,-0.00050918,1.9879,-0.81341,0.58169,0.0001986,140.19,0.00045773,0.00029864,1,14.402
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J341_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.3869, steps = 116
shifted from previous position = 11.6
rotated from previous position = 26.9 degrees
atoms outside contour = 42335, contour level = 0.45548
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J341_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.88671728 0.46231150 -0.00077223 1.30637812
-0.46231114 0.88671760 0.00058820 83.10553465
0.00095669 -0.00016455 0.99999955 12.61810718
Axis -0.00081412 -0.00186986 -0.99999792
Axis point 170.17837953 38.89649216 0.00000000
Rotation angle (degrees) 27.53641478
Shift along axis -12.77454005
> view matrix models
> #1,0.88672,0.46231,-0.00077223,6.8393,-0.46231,0.88672,0.0005882,83.545,0.00095669,-0.00016455,1,79.641
> fitmap #1 inMap #2.1
Fit molecule 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) to map
J341_volume_class_00_map_sharp.mrc (#2.1) using 69260 atoms
average map value = 0.3967, steps = 68
shifted from previous position = 15.5
rotated from previous position = 0.0617 degrees
atoms outside contour = 41304, contour level = 0.45548
Position of 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb (#1) relative to
J341_volume_class_00_map_sharp.mrc (#2.1) coordinates:
Matrix rotation and translation
0.88632938 0.46305324 -0.00140425 1.39488700
-0.46305249 0.88633049 0.00083416 83.23425991
0.00163090 -0.00008910 0.99999868 94.04555879
Axis -0.00099692 -0.00327731 -0.99999413
Axis point 169.55583224 38.81338141 0.00000000
Rotation angle (degrees) 27.58445405
Shift along axis -94.31918182
> volume #2.1 level 0.3598
> select clear
> volume #2.1 level 0.22
> volume #2.3 level 0.169
> select /B:43
24 atoms, 23 bonds, 1 residue, 1 model selected
> select up
63 atoms, 62 bonds, 4 residues, 1 model selected
> select up
5575 atoms, 5617 bonds, 358 residues, 1 model selected
> molmap sel 12 onGrid #2.1
Opened 2DCLK_2Kif3A_fitted_P501_J219_modelled.pdb map 12 as #5, grid size
90,90,90, pixel 3.31, shown at level 0.0518, step 1, values float32
No map chosen to save
> save /Users/tomforan/Library/CloudStorage/OneDrive-
> Birkbeck,UniversityofLondon/references/KifMD_P501J341.mrc models #5
Unsupported scale factor (0.000000) detected on Display0
[Repeated 3 time(s)]An error occurred in drawing the scene. Redrawing graphics
is now stopped to avoid a continuous stream of error messages. To restart
graphics use the command "graphics restart" after changing the settings that
caused the error.
GLError(
err = 1286,
description = b'invalid framebuffer operation',
baseOperation = glBlitFramebuffer,
cArguments = (
0,
0,
2348,
1740,
0,
0,
2348,
1740,
GL_COLOR_BUFFER_BIT,
GL_NEAREST,
)
)
Traceback (most recent call last):
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/core/updateloop.py", line 84, in draw_new_frame
view.draw(check_for_changes = False)
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/view.py", line 188, in draw
self._draw_scene(camera, drawings)
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/view.py", line 278, in _draw_scene
offscreen.finish(r)
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/opengl.py", line 1867, in finish
cfb.copy_from_framebuffer(fb, depth=False)
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/opengl.py", line 2414, in copy_from_framebuffer
GL.glBlitFramebuffer(0, 0, w, h, 0, 0, w, h, what, GL.GL_NEAREST)
File "src/errorchecker.pyx", line 59, in
OpenGL_accelerate.errorchecker._ErrorChecker.glCheckError
OpenGL.error.GLError: GLError(
err = 1286,
description = b'invalid framebuffer operation',
baseOperation = glBlitFramebuffer,
cArguments = (
0,
0,
2348,
1740,
0,
0,
2348,
1740,
GL_COLOR_BUFFER_BIT,
GL_NEAREST,
)
)
Traceback (most recent call last):
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/core/triggerset.py", line 149, in invoke
return self._func(self._name, data)
^^^^^^^^^^^^^^^^^^^^^^^^^^^^
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/sideview/tool.py", line 115, in _redraw
self.render()
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/sideview/tool.py", line 310, in render
self.view.draw()
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/view.py", line 188, in draw
self._draw_scene(camera, drawings)
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/view.py", line 278, in _draw_scene
offscreen.finish(r)
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/opengl.py", line 1867, in finish
cfb.copy_from_framebuffer(fb, depth=False)
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/opengl.py", line 2414, in copy_from_framebuffer
GL.glBlitFramebuffer(0, 0, w, h, 0, 0, w, h, what, GL.GL_NEAREST)
File "src/errorchecker.pyx", line 59, in
OpenGL_accelerate.errorchecker._ErrorChecker.glCheckError
OpenGL.error.GLError: GLError(
err = 1286,
description = b'invalid framebuffer operation',
baseOperation = glBlitFramebuffer,
cArguments = (
0,
0,
1490,
52,
0,
0,
1490,
52,
GL_COLOR_BUFFER_BIT,
GL_NEAREST,
)
)
Error processing trigger "frame drawn":
OpenGL.error.GLError: GLError(
err = 1286,
description = b'invalid framebuffer operation',
baseOperation = glBlitFramebuffer,
cArguments = (
0,
0,
1490,
52,
0,
0,
1490,
52,
GL_COLOR_BUFFER_BIT,
GL_NEAREST,
)
)
File "src/errorchecker.pyx", line 59, in
OpenGL_accelerate.errorchecker._ErrorChecker.glCheckError
See log for complete Python traceback.
Traceback (most recent call last):
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/ui/statusbar.py", line 98, in _expose_event
self.status(self._last_message, self._last_color)
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/ui/statusbar.py", line 164, in status
r.draw_background()
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/opengl.py", line 1238, in draw_background
GL.glClear(flags)
File "src/errorchecker.pyx", line 59, in
OpenGL_accelerate.errorchecker._ErrorChecker.glCheckError
OpenGL.error.GLError: GLError(
err = 1286,
description = b'invalid framebuffer operation',
baseOperation = glClear,
cArguments = (16640,)
)
OpenGL.error.GLError: GLError(
err = 1286,
description = b'invalid framebuffer operation',
baseOperation = glClear,
cArguments = (16640,)
)
File "src/errorchecker.pyx", line 59, in
OpenGL_accelerate.errorchecker._ErrorChecker.glCheckError
See log for complete Python traceback.
Traceback (most recent call last):
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/ui/statusbar.py", line 98, in _expose_event
self.status(self._last_message, self._last_color)
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/ui/statusbar.py", line 164, in status
r.draw_background()
File
"/private/var/folders/2r/qhkfdw750gx2px47fpk_w2br0000gn/T/AppTranslocation/7F62CD4E-C1D2-4027-A589-47AEF404F26A/d/ChimeraX-1.11.1.app/Contents/Library/Frameworks/Python.framework/Versions/3.11/lib/python3.11/site-
packages/chimerax/graphics/opengl.py", line 1238, in draw_background
GL.glClear(flags)
File "src/errorchecker.pyx", line 59, in
OpenGL_accelerate.errorchecker._ErrorChecker.glCheckError
OpenGL.error.GLError: GLError(
err = 1286,
description = b'invalid framebuffer operation',
baseOperation = glClear,
cArguments = (16640,)
)
OpenGL.error.GLError: GLError(
err = 1286,
description = b'invalid framebuffer operation',
baseOperation = glClear,
cArguments = (16640,)
)
File "src/errorchecker.pyx", line 59, in
OpenGL_accelerate.errorchecker._ErrorChecker.glCheckError
See log for complete Python traceback.
Unsupported scale factor (0.000000) detected on Display0
[Repeated 5 time(s)]
OpenGL version: 4.1 Metal - 90.5
OpenGL renderer: Apple M1 Pro
OpenGL vendor: Apple
Python: 3.11.9
Locale: en_US.UTF-8
Qt version: PyQt6 6.9.1, Qt 6.9.0
Qt runtime version: 6.9.2
Qt platform: cocoa
Hardware:
Hardware Overview:
Model Name: MacBook Pro
Model Identifier: MacBookPro18,1
Model Number: MK193B/A
Chip: Apple M1 Pro
Total Number of Cores: 10 (8 Performance and 2 Efficiency)
Memory: 16 GB
System Firmware Version: 18000.120.36
OS Loader Version: 18000.120.36
Software:
System Software Overview:
System Version: macOS 26.5 (25F71)
Kernel Version: Darwin 25.5.0
Time since boot: 11 days, 7 hours, 52 minutes
Graphics/Displays:
Apple M1 Pro:
Chipset Model: Apple M1 Pro
Type: GPU
Bus: Built-In
Total Number of Cores: 16
Vendor: Apple (0x106b)
Metal Support: Metal 4
Displays:
Color LCD:
Display Type: Built-in Liquid Retina XDR Display
Resolution: 3456 x 2234 Retina
Main Display: Yes
Mirror: Off
Online: Yes
Automatically Adjust Brightness: Yes
Connection Type: Internal
Installed Packages:
aiohappyeyeballs: 2.6.1
aiohttp: 3.13.1
aiosignal: 1.4.0
alabaster: 1.0.0
annotated-types: 0.7.0
anyio: 4.12.1
appdirs: 1.4.4
appnope: 0.1.4
asttokens: 3.0.1
attrs: 25.4.0
babel: 2.17.0
beautifulsoup4: 4.13.5
blockdiag: 3.0.0
blosc2: 3.12.2
build: 1.3.0
certifi: 2025.7.14
cftime: 1.6.5
charset-normalizer: 3.4.4
ChimeraX-AddCharge: 1.5.20
ChimeraX-AddH: 2.2.8
ChimeraX-AlignmentAlgorithms: 2.0.2
ChimeraX-AlignmentHdrs: 3.6.1
ChimeraX-AlignmentMatrices: 2.1
ChimeraX-Alignments: 3.1.1
ChimeraX-AlphaFold: 1.0.1
ChimeraX-AltlocExplorer: 1.2
ChimeraX-AmberInfo: 1.0
ChimeraX-Animations: 1.0
ChimeraX-Aniso: 1.3.2
ChimeraX-Arrays: 1.1
ChimeraX-Atomic: 1.61.4
ChimeraX-AtomicLibrary: 14.2.1
ChimeraX-AtomSearch: 2.0.1
ChimeraX-AxesPlanes: 2.4
ChimeraX-BasicActions: 1.1.3
ChimeraX-BILD: 1.0
ChimeraX-BlastProtein: 3.0.0
ChimeraX-Boltz: 1.1
ChimeraX-BondRot: 2.0.4
ChimeraX-BugReporter: 1.0.2
ChimeraX-BuildStructure: 2.13.1
ChimeraX-Bumps: 1.0
ChimeraX-BundleBuilder: 1.6.0
ChimeraX-ButtonPanel: 1.0.1
ChimeraX-CageBuilder: 1.0.1
ChimeraX-CellPack: 1.0
ChimeraX-Centroids: 1.4
ChimeraX-ChangeChains: 1.1
ChimeraX-CheckWaters: 1.5
ChimeraX-ChemGroup: 2.0.2
ChimeraX-Clashes: 2.3
ChimeraX-Clipper: 0.26.1
ChimeraX-ColorActions: 1.0.5
ChimeraX-ColorGlobe: 1.0
ChimeraX-ColorKey: 1.5.8
ChimeraX-CommandLine: 1.3.0
ChimeraX-ConnectStructure: 2.0.1
ChimeraX-Contacts: 1.0.1
ChimeraX-Core: 1.11.1
ChimeraX-CoreFormats: 1.2
ChimeraX-coulombic: 1.4.5
ChimeraX-Crosslinks: 1.0
ChimeraX-Crystal: 1.0
ChimeraX-CrystalContacts: 1.0.1
ChimeraX-DataFormats: 1.2.4
ChimeraX-Dicom: 1.2.7
ChimeraX-DistMonitor: 1.4.2
ChimeraX-DockPrep: 1.1.4
ChimeraX-Dssp: 2.0
ChimeraX-EMDB-SFF: 1.0
ChimeraX-ESMFold: 1.0
ChimeraX-FileHistory: 1.0.1
ChimeraX-FunctionKey: 1.0.1
ChimeraX-Geometry: 1.3
ChimeraX-gltf: 1.0
ChimeraX-Graphics: 1.4.1
ChimeraX-Hbonds: 2.5.3
ChimeraX-Help: 1.3
ChimeraX-HKCage: 1.3
ChimeraX-IHM: 1.1
ChimeraX-ImageFormats: 1.2
ChimeraX-IMOD: 1.0
ChimeraX-IO: 1.0.4
ChimeraX-ISOLDE: 1.11.1
ChimeraX-ItemsInspection: 1.0.1
ChimeraX-IUPAC: 1.0
ChimeraX-KVFinder: 1.7.3
ChimeraX-Label: 1.2
ChimeraX-ListInfo: 1.2.2
ChimeraX-Log: 1.2.2
ChimeraX-LookingGlass: 1.1
ChimeraX-Maestro: 1.9.2
ChimeraX-Map: 1.3
ChimeraX-MapData: 2.0
ChimeraX-MapEraser: 1.0.1
ChimeraX-MapFilter: 2.0.1
ChimeraX-MapFit: 2.0
ChimeraX-MapSeries: 2.1.1
ChimeraX-Markers: 1.0.1
ChimeraX-Mask: 1.0.2
ChimeraX-MatchMaker: 2.2.2
ChimeraX-MCopy: 1.0
ChimeraX-MCPServer: 0.1.0
ChimeraX-MDcrds: 2.17.2
ChimeraX-MedicalToolbar: 1.1
ChimeraX-Meeting: 1.0.1
ChimeraX-Minimize: 1.3.2
ChimeraX-MLP: 1.1.1
ChimeraX-mmCIF: 2.16
ChimeraX-MMTF: 2.2
ChimeraX-ModelArchive: 1.0
ChimeraX-Modeller: 1.5.22
ChimeraX-ModelPanel: 1.6
ChimeraX-ModelSeries: 1.0.1
ChimeraX-Mol2: 2.0.3
ChimeraX-Mole: 1.0
ChimeraX-Morph: 1.0.2
ChimeraX-MouseModes: 1.2
ChimeraX-Movie: 1.0.1
ChimeraX-MutationScores: 1.0
ChimeraX-Neuron: 1.0
ChimeraX-Nifti: 1.2
ChimeraX-NMRSTAR: 1.0.2
ChimeraX-NRRD: 1.2
ChimeraX-Nucleotides: 2.0.3
ChimeraX-OpenCommand: 1.15.2
ChimeraX-OrthoPick: 1.0.1
ChimeraX-PDB: 2.7.12
ChimeraX-PDBBio: 1.0.1
ChimeraX-PDBLibrary: 1.0.5
ChimeraX-PDBMatrices: 1.0
ChimeraX-PickBlobs: 1.0.1
ChimeraX-Positions: 1.0
ChimeraX-PresetMgr: 1.1.4
ChimeraX-ProfileGrids: 1.4.2
ChimeraX-PubChem: 2.2
ChimeraX-ReadPbonds: 1.0.1
ChimeraX-Registration: 1.1.2
ChimeraX-RemoteControl: 1.0
ChimeraX-RenderByAttr: 1.6.5
ChimeraX-RenumberResidues: 1.1
ChimeraX-ResidueFit: 1.0.1
ChimeraX-RestServer: 1.3.3
ChimeraX-RNALayout: 1.0
ChimeraX-RotamerLibMgr: 4.0
ChimeraX-RotamerLibsDunbrack: 2.0
ChimeraX-RotamerLibsDynameomics: 2.0
ChimeraX-RotamerLibsRichardson: 2.0
ChimeraX-SaveCommand: 1.5.2
ChimeraX-Scenes: 0.3.1
ChimeraX-SchemeMgr: 1.0
ChimeraX-SDF: 2.0.3
ChimeraX-Segger: 1.0
ChimeraX-Segment: 1.0.1
ChimeraX-Segmentations: 3.5.10
ChimeraX-SelInspector: 1.0
ChimeraX-SeqView: 2.17.3
ChimeraX-Shape: 1.1
ChimeraX-Shell: 1.0.1
ChimeraX-Shortcuts: 1.2.1
ChimeraX-ShowSequences: 1.0.3
ChimeraX-SideView: 1.0.1
ChimeraX-SimilarStructures: 1.0.1
ChimeraX-Smiles: 2.1.2
ChimeraX-SmoothLines: 1.0
ChimeraX-SpaceNavigator: 1.0
ChimeraX-StdCommands: 1.19.1
ChimeraX-STL: 1.0.1
ChimeraX-Storm: 1.0
ChimeraX-StructMeasure: 1.2.1
ChimeraX-Struts: 1.0.1
ChimeraX-Surface: 1.0.1
ChimeraX-SwapAA: 2.0.1
ChimeraX-SwapRes: 2.5.2
ChimeraX-TapeMeasure: 1.0
ChimeraX-TaskManager: 1.0
ChimeraX-Test: 1.0
ChimeraX-Toolbar: 1.2.3
ChimeraX-ToolshedUtils: 1.2.4
ChimeraX-Topography: 1.0
ChimeraX-ToQuest: 1.0
ChimeraX-Tug: 1.0.1
ChimeraX-UI: 1.49.1
ChimeraX-Umap: 1.0
ChimeraX-uniprot: 2.3.2
ChimeraX-UnitCell: 1.0.1
ChimeraX-ViewDock: 1.5.2
ChimeraX-VIPERdb: 1.0
ChimeraX-Vive: 1.1
ChimeraX-VolumeMenu: 1.0.1
ChimeraX-vrml: 1.0
ChimeraX-VTK: 1.0
ChimeraX-WavefrontOBJ: 1.0
ChimeraX-WebCam: 1.0.2
ChimeraX-WebServices: 1.1.5
ChimeraX-Zone: 1.0.1
click: 8.3.1
colorama: 0.4.6
comm: 0.2.3
contourpy: 1.3.3
coverage: 7.13.1
cxservices: 1.2.3
cycler: 0.12.1
Cython: 3.1.4
debugpy: 1.8.19
decorator: 5.2.1
docutils: 0.21.2
executing: 2.2.1
filelock: 3.19.1
fonttools: 4.61.1
frozenlist: 1.8.0
funcparserlib: 2.0.0a0
glfw: 2.10.0
grako: 3.16.5
h11: 0.16.0
h5py: 3.15.1
html2text: 2025.4.15
httpcore: 1.0.9
httpx: 0.28.1
httpx-sse: 0.4.3
idna: 3.11
ihm: 2.2
imagecodecs: 2024.6.1
imagesize: 1.4.1
iniconfig: 2.3.0
ipykernel: 6.30.1
ipython: 9.5.0
ipython_pygments_lexers: 1.1.1
ipywidgets: 8.1.8
jedi: 0.19.2
Jinja2: 3.1.6
jsonschema: 4.26.0
jsonschema-specifications: 2025.9.1
jupyter_client: 8.6.3
jupyter_core: 5.9.1
jupyterlab_widgets: 3.0.16
kiwisolver: 1.4.9
line_profiler: 5.0.0
lxml: 6.0.2
lz4: 4.3.2
Markdown: 3.8.2
MarkupSafe: 3.0.3
matplotlib: 3.10.7
matplotlib-inline: 0.2.1
mcp: 1.18.0
msgpack: 1.1.1
multidict: 6.7.0
ndindex: 1.10.1
nest-asyncio: 1.6.0
netCDF4: 1.6.5
networkx: 3.3
nibabel: 5.2.0
nptyping: 2.5.0
numexpr: 2.14.1
numpy: 1.26.4
OpenMM: 8.2.0
openvr: 1.26.701
packaging: 25.0
ParmEd: 4.2.2
parso: 0.8.5
pep517: 0.13.1
pexpect: 4.9.0
pickleshare: 0.7.5
pillow: 11.3.0
pip: 25.2
pkginfo: 1.12.1.2
platformdirs: 4.5.1
pluggy: 1.6.0
prompt_toolkit: 3.0.52
propcache: 0.4.1
psutil: 7.0.0
ptyprocess: 0.7.0
pure_eval: 0.2.3
py-cpuinfo: 9.0.0
pybind11: 3.0.1
pycollada: 0.8
pydantic: 2.12.5
pydantic-settings: 2.12.0
pydantic_core: 2.41.5
pydicom: 2.4.4
Pygments: 2.18.0
pynmrstar: 3.3.6
pynrrd: 1.0.0
PyOpenGL: 3.1.10
PyOpenGL-accelerate: 3.1.10
pyopenxr: 1.1.4501
pyparsing: 3.3.2
pyproject_hooks: 1.2.0
PyQt6-commercial: 6.9.1
PyQt6-Qt6: 6.9.2
PyQt6-WebEngine-commercial: 6.9.0
PyQt6-WebEngine-Qt6: 6.9.2
PyQt6_sip: 13.10.2
pytest: 9.0.2
pytest-cov: 7.0.0
python-dateutil: 2.9.0.post0
python-dotenv: 1.2.1
python-multipart: 0.0.21
pyzmq: 27.1.0
qtconsole: 5.7.0
QtPy: 2.4.3
qtshim: 1.2
RandomWords: 0.4.0
referencing: 0.37.0
requests: 2.32.5
roman-numerals: 4.1.0
roman-numerals-py: 4.1.0
rpds-py: 0.30.0
scipy: 1.14.0
setuptools: 80.9.0
sfftk-rw: 0.8.1
six: 1.17.0
snowballstemmer: 3.0.1
sortedcontainers: 2.4.0
soupsieve: 2.8.3
Sphinx: 8.2.3
sphinx-autodoc-typehints: 3.2.0
sphinxcontrib-applehelp: 2.0.0
sphinxcontrib-blockdiag: 3.0.0
sphinxcontrib-devhelp: 2.0.0
sphinxcontrib-htmlhelp: 2.1.0
sphinxcontrib-jsmath: 1.0.1
sphinxcontrib-qthelp: 2.0.0
sphinxcontrib-serializinghtml: 2.0.0
sse-starlette: 3.2.0
stack-data: 0.6.3
starlette: 0.52.1
superqt: 0.7.6
tables: 3.10.2
tcia_utils: 1.5.1
tifffile: 2025.3.13
tinyarray: 1.2.5
tornado: 6.5.4
traitlets: 5.14.3
typing-inspection: 0.4.2
typing_extensions: 4.15.0
urllib3: 2.6.3
uvicorn: 0.40.0
wcwidth: 0.3.2
webcolors: 24.11.1
wheel: 0.45.1
wheel-filename: 1.4.2
widgetsnbextension: 4.0.15
yarl: 1.22.0
Change History (2)
comment:1 by , 3 weeks ago
| Component: | Unassigned → Graphics |
|---|---|
| Owner: | set to |
| Platform: | → all |
| Project: | → ChimeraX |
| Status: | new → assigned |
| Summary: | ChimeraX bug report submission → glBlitFramebuffer: invalid framebuffer operation after Unsupported scale factor detected |
comment:2 by , 3 weeks ago
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Report shows only Mac laptop display was in use. But I suspect ChimeraX was started on an external display, then the display was disconnected, and somehow Qt didn't handle the display disconnect correctly, hence the scale factor 0 warnings that we have seen many times before.
It may be that our OpenGL context is still associated with the disconnected display. This reports shows tracebacks for both our main graphics rendering and statusline rendering. Our OpenGL context code keeps a reference to th QScreen the context was created on. Should check if that code has problems. Should also try starting ChimeraX on external display then unplugging that display.