[Chimera-users] Calculation of the average isoelectric point of a proteins solvation shell

Csaba Nagy csaba.nagy at utu.fi
Fri Nov 20 05:37:34 PST 2015


Dear Chimera Developers and -Users,

I am struggling with the overexpression and purification of a cytoplasmic protein that looks to be soluble only in a very narrow pH range.
I know the average isoelectric point of the whole protein, but there would be much more informative, to know what is the average isoelectric point of the proteins solvation shell, I think. Therefore I modeled the 3D structure of the protein and I tried several other visualization programs to calculate this pI value, but I was not successful.
Could you tell me please, if there is any option to make this estimation in Chimera?
Thank you very much in advance!

Best regards,
Csaba Nagy

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Csaba I. Nagy, PhD
Postdoctoral Researcher,
PharmaCity, Itäinen Pitkäkatu 4 C, 6th floor, room 6049
Molecular Plant Biology
University of Turku
FI-20014 TURKU, Finland
Phone +358 2 333 7914
Fax +358 2 333 8075
Email  csaba.nagy at utu.fi
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