[Chimera-users] fusion proteins
brennal1 at msn.com
Tue Jul 28 12:34:02 PDT 2009
Thanks Elaine for the speedy and thorough response, I will try it as soon as possible. Bren.
> From: meng at cgl.ucsf.edu
> To: brennal1 at msn.com
> Subject: Re: [Chimera-users] fusion proteins
> Date: Tue, 28 Jul 2009 10:10:28 -0700
> CC: chimera-users at cgl.ucsf.edu
> Hi Bren,
> You can create bonds, but only within a single model, so before adding
> the bond you could use the "combine" command, or in the Model Panel,
> the "copy/combine" function to combine two models. Then the bond can
> be added with the "bond" command or the "Build Structure" tool.
> However, there is nothing in this process to prevent you from creating
> something completely ridiculous (physically unreasonable). Chimera
> will not change the relative positions of the structures when you add
> the bond, so *before* combining the models you would need to generate
> the desired position of one structure relative to the other.
> There is a little more detail and some links to the documentation in
> this previous post:
> I hope this helps,
> Elaine C. Meng, Ph.D.
> UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
> Department of Pharmaceutical Chemistry
> University of California, San Francisco
> On Jul 27, 2009, at 6:06 PM, Brennal Pierre wrote:
> > Hi, I would like to know if it possible to create fusion proteins by
> > merging two pdb files, or if it is possible in any other way, thanks
> > Bren.
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