Subject: Re: [nmr_sparky] problem using kr command in AutoAssign
From: eiso
Date: Feb 9, 2011

Previous: 774

amrita bhu2005 wrote:

Dear All, I am trying to use restricted peak picking command (kr) of Auto
Assign. What I am doing is that - first I am picking peaks in 1H-15N HSQC
spectra and creating a peak list for it. And then I am using this kr command
to pick peaks in 3D HNCA spectra using HSQC peaks as reference. But on
obtaining the peak list from the peak option, I am not getting proper values
for the three dimensions (two values are depicting values for the nitrogen
axis and one for hydrogen whereas I am expecting the values for N,H and C
axis). Kindly suggest me the steps to use the kr command.

Could it be that the header of your *.ucsf file is incorrect?
[if you use a ucsf file]

use:
% ucsfdata hnco.ucsf
to check if the header has the right nuclei for each dimension.
you can also use this program to correct it.

goodluck, Eiso

Thanking You.

Navdeep Gogna, Amrita Kumari IISER, Mohali, Chandigarh