Subject: Peak picking stratagy
From: caesaregyptcrypt
Date: Apr 2, 2018

Next: 1320


So I am trying to solve the structure of a protein construct that is very similar to one published in the BMRB. The only difference is 13 extra residues added onto the N-terminus. I have collected HN-HSQC, HNCACB, and CACB(CO)NH spectra on labeled samples and have good peak separation. However, when I use APES peak picking like shown in the tutorial videos, I get no peaks selected in the CBCA(CO)NH spectrum even after relaxing the the picking tolerances significantly. The other two spectra pick some peaks but not all of them. What does the APES routine do exactly and would it be better to simply pick the HSCQ peaks manually and then use the restricted peaking (kr) command on the other two spectra? What is the best practice for this? Thanks for any help you can give.
Best,
David O.