[Chimera-users] Molecular weight of an EM map
goddard at sonic.net
Tue Feb 8 15:45:41 PST 2011
Use the "Measure Volume and Area" dialog under menu Tools / Volume
Data to show the volume in cubic Angstroms enclosed by your map contour
surface. It will update automatically as the threshold changes. Make
sure the voxel size of your map is set correctly in Angstroms because
the reported enclosed volume uses that. That is set in the Volume
Viewer dialog under the Coordinates panel. To estimate molecular weight
from the volume you could use an approximate value for globular proteins
from the literature of 1.21 cubic Angstroms per Dalton (Harpaz 1994).
> Hi Tom,
> I have a question to ask you.
> I'm working on a 3D EM map from single particle reconstuction using Chimera. I
> would like to know the total volumn of this 3D model or its molecular weight
> when I change thresholds. How can I do it or where can I find this value?
> Best regards
> Pu Qian
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