No, i have only a spectrum with a mixing time of 300ms.
--- In nmr_sparky@yahoogroups.com , Zhi Liu liufanghe@... wrote:
Did you do the build up with different mixing time?Jay
From: jennig148 claudia.gerlach@...
To: nmr_sparky@yahoogroups.com
Sent: Friday, February 10, 2012 7:24 AM
Subject: [nmr_sparky] distance constraints from NOESY crosspeaks
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Hello users,
I have a NOESY spectrum of a DNA/RNA strand. I try to get the distance constraints by integration of the NOESY crosspeaks and then converting the volumes into distances.
For calibration i want to use the C H5-H6 crosspeak intensity (2.5 A).
I found the following equation in one message:
distance = 2.5 * (intensity of peak/intensity of C H5-H6 peak) ^ (1/6)
But most of the distances are coming out are too short.
Another equation i found was:
distance =(intensity of peak^(-1/6)-A)*C
But here i dont know what A and C is.
So the question is: What is the correct equation to calculate the distances?
Thanks