Subject: distance constraints from NOESY crosspeaks
From: jennig148
Date: Feb 10, 2012
Next: 814
Hello users,
I have a NOESY spectrum of a DNA/RNA strand. I try to get the distance constraints by integration of the NOESY crosspeaks and then converting the volumes into distances.
For calibration i want to use the C H5-H6 crosspeak intensity (2.5 A).
I found the following equation in one message:
distance = 2.5 * (intensity of peak/intensity of C H5-H6 peak) ^ (1/6)
But most of the distances are coming out are too short.
Another equation i found was:
distance =(intensity of peak^(-1/6)-A)*C
But here i dont know what A and C is.
So the question is: What is the correct equation to calculate the distances?
Thanks