Subject: 回复：Re: [nmr_sparky] a utomated peaks picking
From: wenkai zhu
Date: Jun 15, 2018

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Hi,Woonghee

I have got your point and you really helped me a lot. I still have a question about negtive peak picking since most spectras of macromolecules
are always positive, so can i exclude the negtive peaks? Thank you.


Cheers,
Wen-Kai Zhu


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2018 年 6 月 15 日周五下午 7:03，Woonghee Lee woonghee791113@... [nmr_sparky]
nmr_sparky@yahoogroups.com 写道：

Hi,

When you use kr for TOCSY or NOESY using HSQC eaks, you should empty one tolerance column for the dimension that you do not want to restrain (e.g. indirect H or C).
The version out there supports HCCH-TOCSY not the CCH-TOCSY I guess, but the one will be out supports all.

Woonghee

On Fri, Jun 15, 2018, 3:22 AM wenkai zhu zhuwenkai93@... [nmr_sparky] nmr_sparky@yahoogroups.com wrote:

Dear Woonghee,


Thank you. I checked my HBHA（CO）NH spectrum and solved the problem. But i found that APES didnt work for CCH-TOCSY. As you suggested, i used “kr” command to pick peaks and obviously not all peaks were picked in CCH-TOCSY, the case is same with 13C/15N NOESY.
I guess i didnt use “kr” properly and im looking forward to your reply.

Cheers,

Wen-Kai Zhu


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