Subject: Re: Side chain assignment of small peptide.
From: woonghee_lee
Date: Nov 23 4:37 PM
Previous: 1281 Next: 1283
Dear Sohee,
You can try either automatic and manual, or hybrid approach.As your protein is so small, I think you can just try PINE. If your C-HSQC is constant time, you will get very accurate results with PINE.3. Just type ep in the NMRFAM-Sparky and add your experiments, and click submit. To bootstrap your current assignments to enhance the results, enable Use pre-assignment.
1. Use of PINE-SPARKY.2 plugin for automated assignment
2. PINE-SPARKY extensions for validation
Let me know if you need further help.
Best,
Woonghee