[Chimera-users] on mrc map

Elaine Meng meng at cgl.ucsf.edu
Wed Sep 9 09:30:40 PDT 2015


Dear Smith,
This is a broad question, with different approaches possible depending on the data and how much is known about it.  General possibilities are:
 
(A) segment the map, use segmentation surfaces to mask map to create new maps (see choices in the File menu of Segment Map)
<http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/segger/segment.html>

(B) fit atomic structures, or if no atomic structures, place markers into the four areas, color each structure or marker differently, use Color Zone to color nearby map to match them and then split map by color
<http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/colorzone/colorzone.html>

Each takes several steps and requires interaction and judgment, i.e. I can’t just give a recipe that exactly produces the desired result. I hope this helps,
Elaine
-----
Elaine C. Meng, Ph.D.                       
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco

On Sep 8, 2015, at 6:54 PM, Smith Liu <smith_liu123 at 163.com> wrote:

> Dear All,
> 
> Suppose I have a mcr map for a protein of 4 subunits. Will you please introduce to me how to split the whole map to 4 different maps, with each map corresponding to the part of each subunit in the whole map, without loosing any data in the spitted map  in comparison to that specific subunit part of the whole map?
> 
> Best regards.
> 
> Smith




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