[Chimera-users] Align and average intensity images in Chimera

許紹君 peggyschsu at gate.sinica.edu.tw
Mon Sep 7 17:08:41 PDT 2015


To whom it may concern,

 

I am willing to use Chimera for aligning several images from the structure
illumination microscope (SIM).

Here is the surface maps generated by Chimera from two images. 

cid:image005.jpg at 01D0E986.2CF5F370

 

After alignment by the tool “Fit in Map”, they are aligned very well.

cid:image006.jpg at 01D0E986.2CF5F370

 

My next step is to average the intensity from two aligned images and
generate a new map which will be used to fit other images.

Can anyone tell me how to do it in Chimera? 

By the way, please also tell me what the values mean in the reply log. 

cid:image007.jpg at 01D0E986.2CF5F370

 

It helps me to use another software to complete the mission, if I can get
the rotation, voxel shift, or even scaling values from the alignment.

Thank you!

 

 

 

 

 

All Best,

Peggy Hsu, PhD

Institute of Cellular and Organismic Biology, Academia Sinica

128 Academia Rd Sec 2, Nankang, 

Taipei 11529, Taiwan

Tel: 886-2-2787-1531 (O)

email: peggyschsu at gate.sinica.edu.tw

 

 

 

 

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