[Chimera-users] joining two peptides or proteins

Elaine Meng meng at cgl.ucsf.edu
Mon Jun 22 10:06:41 PDT 2015


Hello,
I’m guessing you actually want to form a bond between the two.  If you have opened the two structures from two different files (so that they are separate models), you can connect the N-term of one peptide or protein to the C-term of the other using the Join Models section of Build Structure (menu: Tools… Structure Editing… Build Structure, change to the Join Models section of that dialog, chose “C-N peptide bond” option).  For the full description and instructions see:
<http://www.rbvi.ucsf.edu/chimera/docs/ContributedSoftware/editing/editing.html#join>

First you should change the display from ribbons to atoms (for example, menu: Presets… Interactive 2 (all atoms)) so that you can select just the two backbone atoms to be joined, the C atom of the C-terminal residue of one protein and the N atom of the N-terminal residue of the other.  
<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/selection.html#pickselect>

I hope this helps,
Elaine
----------
Elaine C. Meng, Ph.D. 
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco


> On Jun 22, 2015, at 5:40 AM, Sezer Akgöl <akgolsezer at gmail.com> wrote:
> 
> how can I associate 2 protein with each other from their C and N terminals respectively




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