[Chimera-users] Coloring an specific area along a morphing serie among several maps

Elaine Meng meng at cgl.ucsf.edu
Tue Aug 4 11:57:56 PDT 2015


Dear Ana-Lucia,
You would probably need a session with the 6 density maps, as well as a colored marker set previously created for each map.  Then, you could use “vop morph” commands to play the map morph, “modeldisp” and “~modeldisp” to show and hide marker sets by their model numbers (since marker sets are implemented as molecule models), and “scolor zone” for coloring zones to match the markers.

<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/vop.html#morph>
<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/midas/scolor.html#zone>

I am imagining something like showing the first map and its markers and color zones, hiding markers and showing morph 1->2, then showing map 2 and its markers and color zones, hiding markers and showing morph 2->3, etc..  

Perhaps you were thinking of showing markers and colors the whole time and having them also morph, but I don’t know if that is possible.  Although there is also a “morph” command to morph between sets of atom coordinates, it is meant for use with biopolymer chains (proteins, DNA, RNA) and it may not work for marker sets.  If you try, here is a previous post about synchronizing map morphing with atom morphing:
<http://plato.cgl.ucsf.edu/pipermail/chimera-users/2013-April/008752.html>

As you may know already, there are lots of other things you can do in the script, like rotating, zooming, and showing title text:
<http://www.rbvi.ucsf.edu/chimera/docs/UsersGuide/movies.html#content>

I hope this helps,
Elaine
----------
Elaine C. Meng, Ph.D. 
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco



> On Aug 4, 2015, at 12:54 AM, Ana Lucia Alvarez Cabrera <anlualca at yahoo.com> wrote:
> 
> Dear Srs,
> 
> I made a chimera movie where six different conformations of the same complex are morphed in a sequence, changing on to the next one (the aim is  showing a conformational change occurring in a number of steps, in this case the six electron microscopy maps).  My complex has an specially flexible domain   and  I need to highlight this area along the morphing sequence of the six maps. I suppose that this can be done using the markers and color zone options, but I don't  know how to implement this orders in the .cmd script of my movie.  Thanks in advance for your help.
> 
> Sincerely,
> 
> Ana-Lucia
> 




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