[Chimera-users] em fit refinement

Feixia fvy2 at unh.edu
Mon Jan 14 14:38:44 PST 2013


Hi Tom,

I have a question about multiple fit.  Essentially, I have a map of a  
dimer.  For each monomer, I have three domains.  I have built models for  
each of the domain.  I tried to fit each individual domain into the map,  
but found the same domain taking different orientations in different  
monomer, so I can't achieve 2-fold symmetry.  I wonder what would be a  
sensible approach to fit.  Also, what would be a good value to select for  
'level' for the map.  The map is from negative-staining data with 18A  
resolution.  Thanks a lot!

Best,
Feixia

-- 
Feixia Chu, Ph.D.
Assistant Professor
Biochemistry Graduate Program Coordinator
Molecular, Cellular & Biomedical Sciences
University of New Hampshire
Gregg Hall, Rm436
35 Colovos Rd
Durham, NH 03824
Tel 603 862 2436


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