[Chimera-users] Disulfide bonds
meng at cgl.ucsf.edu
Tue Dec 4 15:43:10 PST 2012
There are several different ways... I'll mention a few. You could select the two atoms from the Chimera window (Ctrl-click one, Shift-Ctrl-click the other) and then use command:
The Command Line can be shown using the Favorites menu. If the Cys atoms are not already displayed, try command:
Or instead of selecting, you could specify atoms by name, something like:
bond :25.a at sg :45.a at sg
... if the Cys were residue 25 and 45 in chain A.
Or, instead of commands you could use Build Structure (in menu under Tools... Structure Editing), the Adjust Bonds tab.
I hope this helps,
Elaine C. Meng, Ph.D.
UCSF Computer Graphics Lab (Chimera team) and Babbitt Lab
Department of Pharmaceutical Chemistry
University of California, San Francisco
On Dec 4, 2012, at 7:12 AM, Moritz Bitzhenner wrote:
> Dear Chimera Users
> I am fairly new to the program and I have a particular problem with the display of disulfide bonds. I have an amino acid sequence, I created a pdb-file with Expasys Swiss Model Workspace. I opened the pdb file with Chimera and coloured the ribbon structue (it looks amazing), I even did a movie of my rotating protein. But I cannot display the three disulfide bonds.
> Is my pdb file lacking the CONECT information? How can I include them? With Swiss PDB Viewer (Deepviewer) I can show the bonds, but somehow I cannot transfer the information to the pdb file and Chimera.
> I also tried manually bonding the closest cysteins by selecting Cystein residues and "bond" in the command line. How do I select the two single atoms that I want to connect?
> I read through former similar questions in the list server but nothing helped so far.
> If you can find any time helping my case, it would be greatly appreciated.
> With best regards from Germany, Moritz
> Here is the AA sequence:
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