Subject: Re: [nmr_sparky] RH command
From: Josh Ward
Date: Mar 28, 2006

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RH does curve fitting, not integration, so I hope I am following along
alright. If the fitting is being terribly slow, try fitting fewer
peaks. If you have bad data it will take the computer longer to fit,
and if it is an older workstation it will be painful.

Are you trying to fit the data using peak heights or volumes? Here we
find that peak heights are better behaved than volumes. You should
check the integration of each peak carefully, viewing the slices to make
sure there arent any bad fits. That could be slowing you down quite a
bit as well.

Hope that starts to help,

Josh

Tiago Pais wrote:

Hi,
I have a series of HSQC spectra that I am using to calculate
relaxation parameters for my protein.
However, when I use the RH command to integrate the peaks in the
series of spectra, the program stalls after the 10th peak!!I have
more than 100 peaks to integrate.
I am using the spectra in the ucsf format and I load all the spectra
I want to integrate. Then I load the peak list in the firs and run
the RH command with all the parameters chosen in the setup menu of
the RH box.
What am I doing wrong?Why does the program stalls?

Thank you in advance

Tiago

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Josh Ward
Graduate Research Assistant
Purdue University
Department of Medicinal Chemistry and Molecular Pharamacology
Lily Hall of Life Sciences
Phone: (765) 494-2191