Subject: R: [nmr_sparky] Regarding processing problem of 2D TOCSY ucsf in sparky
From: Marco Sette
Date: May 9, 2013

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Hi Pancham,

did you check the acquisition parameters you used to record the spectrum?
Best,
Marco

Dr.Marco Sette, Ph.D.

Department of Chemical Sciences and Technology
University of Rome, Tor Vergata
via della Ricerca Scientifica, 00133, Rome, Italy
e-mail: sette@...
e-mail: m77it@...
Tel.: +39-0672594424
Fax: +39-0672594328

www.rete29aprile.it

--- Gio 9/5/13, pancham kandiyal_mpharm@... ha scritto:

Da: pancham kandiyal_mpharm@...
Oggetto: [nmr_sparky] Regarding processing problem of 2D TOCSY ucsf in sparky
A: nmr_sparky@yahoogroups.com
Data: Giovedì 9 maggio 2013, 06:33

Dear sir/Madam,
I am a sparky user and using this software for 2D NMR assignment....I used this software many time...I am facing a problem in ucsf file of 2D tocsy NMR....my sample is in CDCl3 and I fixed the TMS at 0 ppm on both W1 and W2 axis...but if I put the cursor on any other contour of diagonal of this 2D tocsy spectra both the W1 and W2 value are not same....... only that diagonal contour is showing same W1 and W2 value which is fixed by ST command for TMS at 0 ppm.....Same way I did 2D roesy TMS adjustment at 0 ppm...and this case is fine....Due to the problem in tocsy I am unable to synchronize both the 2D tocsy and roesy NMR data...so I am unable to understand the problem...please help me to solve this problem so I can work further....

Thankyou

Regards
Pancham Kandiyal
CDRI, Lucknow
India