Subject: Re: [nmr_sparky] sparky relaxation fit problem
From: homespuner
Date: Sep 13, 2012

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Hi Tom,

If I understand correctly, the unit of T2 in your case is mini-second (120 ms = 0.12 s). Therefore the R2 in your example is 1/0.12 = 8.33 s-1.

Kuen-Phon Wu

On Thu, Sep 13, 2012 at 5:37 PM, webtrekus webtrekus@... wrote:
 

Dear sparky users,
I am trying to get r1 and R2 values
I compared to nmrview. the results from sparky is 100 times smaller that the results I got from nmrview. I dont know what is wrong.
If somebody could help, that will be great.

the following is what I did:

precess the data using nmrpipe, so I can use it in nmrview and sparky (pipe2ucsf out.ft2 out.ucsf).

I picked peaks, and type pc, then pi, fowlloing that I type oc and op to copy all the assigments to other spectra.
the I go the first spectra. type rh the did set up, the input time parameters are 11.2, 22.4, 44.8, 67.2, 89.6. 22.4 and 67.2 (in ms).
then I type rh again, it give me the value which is (T2) about 120, which indicates that R2 will be around 0.01( s-1).
However I also run nmrview, and get intensities, then fit, I got the R2 values around 10-15 (s-1), which is reasonable.

Can somebody tell me why sparky result is much smaller?

I used exact spectra processed by NMRpipe, so the data are exact same.

Thank
Tom