--- In nmr_sparky@yahoogroups.com , Zhi Liu liufanghe@... wrote:

Did you do the build up with different mixing time?Jay

From: jennig148 claudia.gerlach@...

To: nmr_sparky@yahoogroups.com
Sent: Friday, February 10, 2012 7:24 AM
Subject: [nmr_sparky] distance constraints from NOESY crosspeaks


 
Hello users,

I have a NOESY spectrum of a DNA/RNA strand. I try to get the distance constraints by integration of the NOESY crosspeaks and then converting the volumes into distances.

For calibration i want to use the C H5-H6 crosspeak intensity (2.5 A).
I found the following equation in one message:

distance = 2.5 * (intensity of peak/intensity of C H5-H6 peak) ^ (1/6)

But most of the distances are coming out are too short.

Another equation i found was:

distance =(intensity of peak^(-1/6)-A)*C

But here i dont know what A and C is.

So the question is: What is the correct equation to calculate the distances?

Thanks