Subject: vnm2ucsf issues
From: Gilles Pics
Date: Aug 29, 2011

Next: 804


Hello

Im experiencing issues when I run vnmr2ucsf, especially on 2D 1H/15N HSQC spectra. After conversion of my Varian spectra to the ucsf format, all the peaks are shifted in the 15N dimension by 0.5ppm (comparison between a spectrum displayed in Vnmr and Sparky).
Im carefully using the recommended commands (trace=f1, display the full spectrum, and use the VNMR flush command) in Vnmr before running vnmr2ucsf.
I noticed that the shift in the 15N dimension is dependent on the processing parameter used such as the zero-filling level.

Can anyone help me?

Thanks a lot