Subject: Processing 2D version of 4D 15N,15N-hsqcnoesyhsqc
From: abhishek
Date: May 24, 2011



Hello

I am having problem processing a 2D version (both proton dimension) of a 4D 15N,15N-hsqcnoesyhsqc experiment obtained on varian600. When I tried to convert the data to pipe and read the fid, it only recognized the direct dimension. So I choose 2D dimension from the options given and processed it (below). The diagonal peaks on the processed spectrum appears folded. I am not sure why that is since I used a large spectral window based on chemical shifts from a 2D noesy and the peaks should not fold.

Any help would be greatly appreciated.

Thanks

Abhishek

#!/bin/csh

var2pipe -in ./fid
-noaswap
-xN 1360 -yN 200
-xT 680 -yT 100
-xMODE Complex -yMODE Complex
-xSW 8000.000 -ySW 6000.150
-xOBS 599.766 -yOBS 599.766
-xCAR 4.773 -yCAR 4.773
-xLAB HN -yLAB H1
-ndim 2 -aq2D States
-out ./test.fid -verb -ov

sleep



#!/bin/csh

#
# Basic 2D Phase-Sensitive Processing:
# Cosine-Bells are used in both dimensions.
# Use of ZF -auto doubles size, then rounds to power of 2.
# Use of FT -auto chooses correct Transform mode.
# Imaginaries are deleted with -di in each dimension.
# Phase corrections should be inserted by hand.

nmrPipe -in test.fid
| nmrPipe -fn SOL
| nmrPipe -fn SP -off 0.5 -end 1.00 -pow 2 -c 1.0
| nmrPipe -fn ZF -auto
| nmrPipe -fn FT -auto
| nmrPipe -fn PS -p0 224 -p1 0.00 -di -verb
| nmrPipe -fn TP
| nmrPipe -fn SP -off 0.5 -end 1.00 -pow 1 -c 1.0
| nmrPipe -fn ZF -auto
| nmrPipe -fn FT -auto
| nmrPipe -fn PS -p0 87 -p1 0.00 -di -verb
-ov -out 2D_KDO.ft2