Subject: Re: Pseudo 3D T2_relaxation
From: Tom Goddard
Date: Feb 23, 2011

Next: 780

Hi Mohammad,

Sparky has no capability to treat a 3-d spectrum as a stack of 2-d
spectra. So even if you succeed at getting this in UCSF format it wont
be useful. Sparkys ability to fit relaxation data requires separate
files for each 2-d spectrum. The pipe2ucsf program was only written to
handle real 2-d, 3-d, and 4-d spectra and probably cannot understand
some aspect of the nmrPipe format of 3-d stacks of 2-d spectra.

Best to post Sparky questions to the nmr_sparky discussion group.

http://tech.groups.yahoo.com/group/nmr_sparky/

Tom

Hello

I have collected T2 relaxation experiment in a pseudo 3D format where
axes 1 and 3 correspond to proton and nitrogen and axes 2 corresponds
to different relaxation times (11 relaxation times).

During processing of the spectra with nmrpipe, I partition the
pseudo-3D in to 11 parts each corresponding to one relaxation time.

I can open the .ft2 files in nmrDraw and they look fine.

But when I try to convert them to .ucsf files using pipe2ucsf
command, it complains that the file is smaller than expected size by a
factor of 11.

Could you let me know how I can convert them to .ucsf files for Sparky.

Regards,
Mohammad.