| Hi, I think you should read something but in general (I have no Sparky open in this moment): -the N15 axis is your z-axis, so you move into the planes changing the N15 axis, I think the w3 you mention but I donthave Sparkyavailable now. -with N15 spectra only you use the classical Wuthrich approach and the N15 avoid overlaps -paksat the top of your HSQC are probably (not 100% sure)glycines and you can confirm this looking at the N15-tocsy.This are good starting points -in general you could avoid starting points: after picking selectisolated peaks in the HSQC and use the sp to find neighboursusing the Wuthrichs rules bye Marco --- Mer 9/6/10, Maria Tareen mariatareen27@... ha scritto:
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