Subject: peak intensities for steady state noe
From: Tom Goddard
Date: May 6, 2009

Next: 585


Hi Veal,

Peak volumes or heights can be easy to get and accurate or hard and
unreliable -- depends what your spectrum is like.
Easy if the peaks are not overlapped and baselines are flat at zero.
You always have to see if you are getting reliable numbers. If you are
fitting the peaks look at the 1-d slices (vS) which show both the
experimental (black) and fit curve (blue).

It is very unlikely that using Python code will help you in any way
get reasonable peak volumes or heights if the standard Sparky user
interface cant do it.

Tom


veal wrote:

Dear Sparky developer

I have to get the intensities of steady-state noe spectra. I heard
from other user that old version of Sparky has problems in reading the
exact intensities of steady-state noe spectra because the selection of
base line level. Is that true or it just a rumor? If it is true, is it
fixed? Or I have to use the python scripts to do so? I have used
Sparky for assignment for several years but I never used python. Do I
have to know anything else before using Sparky to get the intensities
of steady-state noe spectra? Or I can just trust the intensities it
give me as for any other spectra.



Best regards,



Veal Liu