Subject: about spin graph assigner used in hcch-tocsy assignment
From: lithlin
Date: Jul 24, 2007

Next: 409


It seems like cch-tocsy is not supported by spin graph
assigner(shortcut ga), so i mannually assigned the side-chain carbon
atoms using cch-tocsy. But after this when i tried to assign
the hcch-tocsy using spin graph assigner, it never gave me the proper
chemical shifts of a side-chain proton. I read the description of
spin graph assigner, here is what i figured out. When i click a
side-chain proton in spin graph, it searches the chemical shift of the
carbon atom the proton attached using the spectrum it is provided,
like cconh which is supported. but as all the carbon atom(except CA,
CB) are assigned using cch-tocsy which is not supported, the chemical
shift of carbon atom is not available. so the candidate chemical
shifts of the proton can not be appropriately provided. Is what i
said above right? If it is, why doesnt the assigner get the chemical
shifts of carbon atoms directly from the assignment table. If i am
wrong, please help me out