Subject: HBCBCGCDHD experiment
From: jleeweir
Date: Nov 30 4:59 AM



Hi to all,

Recently I wanner confirm the aromatic side-chain through HBCBCGCDHD
experiment, but the spectrum i got is much different from whats in the
article of Lewis E. Kay on JACS in 1993.

First, Y and F always have two different HDs in structured domain,
while in the article only one HD or HE can be seen from the spectrum.
Second, about the time it needs to have a good spectrum.I collected a
spectrum in two hrs on a Varian UNITY 600-MHz with 1 mM protein in D2O
and the peaks are less than it should be.

Thanks!

jleeweir