Dear Sparky Users,

I initially ran CYANA using peaks that were well defined (no overlaps) in the 15N-edited and 13C-edited spectra. After cycle7, the CYANA run generates cycle7-ref.peaks and cycle7.peaks. I input these files in SPARKY and tried using the command rp so that SPARKY reads the peak lists, but it doesn’t work. Is there a way by which these files can be read by SPARKY so that I need not manually type and assign the peaks in the spectra? Also, my next CYANA run would include peaks that are overlapped. When these peaks are integrated I feel SPARKY is overestimating the peak volumes. How do you guys handle this problem?

Thanks,

Betty Swanson