Subject: Re: [nmr_sparky] problems with peak picking
From: Mandar T. Naik
Date: Jun 5, 2006

Previous: 155

I am amazed that kr didnt give you expected results.

In case you are working with a unstable protein sample, like mine :(, where
peaks get moved slightly with time. (As al will only calibrate single peak
while other peaks might be deviated from their position in HSQC and thus are
not detected by kr). I recommend you to prepare 2D projection of HNcoCA and
tally it with HSQC. Use:
1. ucsfdata HNcoCA.ucsf
Note down the matrix size (X) for 13C
2. ucsfdata -s1 X -r -o HNcoCA_23.ucsf HNcoCA.ucsf
In sparky ol HSQC.ucsf and HNcoCA_23.ucsf,
Best regards
-mandar

On Tuesday 06 June 2006 08:57, jpwml wrote:
Thanks a lot for the advise, the
method with choosing world and then peak picking seems to work fine.
I will test it in more detail today.

(This should work but in case it didnt go to rt, switch to F8,
select world and apply mode.)

Ciao

Lassalle