Subject: Re: [nmr_sparky] Question About EasyBruk2UCSF Utility
From: Eiso AB
Date: Feb 4, 2019

Previous: 1448

Hi James,

you can also supply the value of nc_proc when you run xfb, like in:

xfbnc_proc 2


This example scales down the data twice. However, you can only scale the data
more down**

(or less up) than the command would have done without the argument *nc_proc*


So find the largest nc_proc in your series and apply them to all planes.


Goodluck,


Eiso



Woonghee Lee woonghee791113@... [nmr_sparky] wrote:
Dear James,

I think that is NC_proc parameter related scaling problem processed in TopSpin.
When you process your data in TopSpin, intensities will be relatively adjusted
based on NC_proc parameter.
1. You can open procs file with a text editor like wordpad, find ##$NC_proc=
and edit them to have all same values (e.g. -8). Then, run easy_bruk2ucsf.
2. Another way is just to run bruk2ucsf with NC_proc parameter on
terminal. Syntax: bruk2ucsf bruker-file ucsf-file opt: NC_proc

Best,
Woonghee

On Mon, Feb 4, 2019 at 9:33 AM Gibson, James gibsoj3@...
mailto:gibsoj3@... [nmr_sparky] nmr_sparky@yahoogroups.com
mailto:nmr_sparky@yahoogroups.com wrote:

Starting from Topspin, I had a pseudo 3D experiment.  I repeatedly used
xfb to get all my 2D planes. Then, when I use the bruk2ucsf utility from
the NMRFAM pulldown menu in nmrfam_sparky, I see command lines such as:

/[DataLocation]/102/2rr/101.uscsf|-8
/[DataLocation]/102/2rr/102.uscsf|-9
/[DataLocation]/102/2rr/103.uscsf|-8

etc. But sometimes, I have 34 slices and about 30 of them have the -8, and
then only 4 have the -9, and then -9 2D files are significantly higher in
intensity across the board for peaks I know should be the same height in
all of them.  What does this final number mean and is there any way to
change it?

Thanks,

James
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