Hello,

I am a new sparky user, using it through NMRbox. I have 3D backbone spectrathat I processed using NMRFx and am able to visualize and align with the corresponding 15N HSQC. I would like to use APES to assist my peak picking. I have the .seq file and the spectra aligned., but APES returns with no peaks picked. I can see it pick the peaks through the 15N HSQC andwhen it finishes it erases the ones that were picked, also erasing the peaks I picked to align the spectra. I have tried this with a set of 3Ds and also just one or two of them to get the same result.

Any suggestions to fix this?

Thank you for your time.