On Fri, Jun 15, 2018 at 10:02 AM, wenkai zhu zhuwenkai93@... [nmr_sparky] nmr_sparky@yahoogroups.com wrote:Hi,Woonghee
I have got your point and you really helped me a lot. I still have a question about negtive peak picking since most spectras of macromoleculesare always positive, so can i exclude the negtive peaks? Thank you.Cheers,Wen-Kai Zhu
2018 年 6 月 15 日周五下午 7:03，Woonghee Lee woonghee791113@... [nmr_sparky]Hi,When you use kr for TOCSY or NOESY using HSQC eaks, you should empty one tolerance column for the dimension that you do not want to restrain (e.g. indirect H or C).The version out there supports HCCH-TOCSY not the CCH-TOCSY I guess, but the one will be out supports all.Woonghee
On Fri, Jun 15, 2018, 3:22 AM wenkai zhu zhuwenkai93@... [nmr_sparky] nmr_sparky@yahoogroups.com wrote:Dear Woonghee,
Thank you. I checked my HBHA（CO）NH spectrum and solved the problem. But i found that APES didnt work for CCH-TOCSY. As you suggested, i used “kr” command to pick peaks and obviously not all peaks were picked in CCH-TOCSY, the case is same with 13C/15N NOESY..I guess i didnt use “kr” properly and im looking forward to your reply.Cheers,Wen-Kai Zhu