Dear All,

Happy holidays. I am attempting to peak pick with APES (NMRFAMUtilitiesAutomated peak picking by APES). I have several spectra including HSQC (N), HSQC (C), NHCO, HNCA, HNCACB, HNCOCA, NOESYHSQC, HNCACO, and CBCACONH. The sequence file seems to read fine, I see the correct sequence in the window when I do sq. When I click Run APES I receive this error message:

Traceback (most recent call last):

File /usr/local/src/nmrfam-sparky-linux/python/lib-tk/Tkinter.py, line 1489, in __call__

return self.func(*args)

File /usr/local/src/nmrfam-sparky-linux/python/sparky/apes.py, line 380, in pick_cb

self.make_sequence_filter()

File /usr/local/src/nmrfam-sparky-linux/python/sparky/apes.py, line 216, in make_sequence_filter

atms = atomnames.protein_atoms_by_group_2[seq]

KeyError: X

Does anyone have any idea what the problem could be?

Thanks a lot,

Liz