For 3D TOCSY or COSY, you will want to see HX from magnetization transfer to be on vertical axis, while CX is put to the Z and attached HX to be horizontal. It is just my personal preference and there may be people want to make a strip horizontally elongated, too.

On Nov 23, 2017 6:42 PM, woonghee791113@... [nmr_sparky] nmr_sparky@yahoogroups.com wrote:

Dear Sohee,

You can try either automatic and manual, or hybrid approach. As your protein is so small, I think you can just try PINE. If your C-HSQC is constant time, you will get very accurate results with PINE.3. Just type ep in the NMRFAM-Sparky and add your experiments, and click submit. To bootstrap your current assignments to enhance the results, enable Use pre-assignment.

1. Use of PINE-SPARKY.2 plugin for automated assignment

http://www.nmrfam.wisc.edu/ pine-sparky2.htm

2. PINE-SPARKY extensions for validation

http://pine.nmrfam.wisc.edu/ PINE-SPARKY/sparky_extensions. html

Let me know if you need further help.

Best,

Woonghee