Subject: Re: [nmr_sparky] 3D HNCA --> 2D Plane with " ucsfdata " and mirroring HSQC plane alone 1H.
From: Eiso AB
Date: Sep 20, 2017

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Whoonghee is pointing you to the right page, but it seems to
me that its the 15N dimension that is flipped, not the 1H.

Try with and without -neg in the FT statement for the 15N dimension, one of them should be correct. (remove -auto if both give
the same result)

Negating imaginary data before Fourier transform is equivalent to applying the REV function followed by a one-point circular shift:

| nmrPipe -fn FT -neg

is equivalent to:

| nmrPipe -fn FT | nmrPipe -fn REV -sw | nmrPipe -fn CS -rs 1 -sw

goodluck, Eiso

Vasantha Kumar M. V. vin.vasanth@... [nmr_sparky] wrote:

Dear Sparky group,

I have a 3D HNCA spectra (15N13C2H sample with deuterium decoupling) acquired on VNMRJ800.
I use the NMRpipe to convert the data into ucsf format. When I loaded ucsf file to sparky the third
dimension (Ca) looks correct, but the HSQC plane (of HNCA) shows the mirroring of the entire spectra alone
1H. I have tried to use xr & xx commands to change the dimensions, found that dimensions are correct,
but all the resonances in HSQC plane flipped horizontally or 180° on 1H axis.
I dont know why.!!!

The script that I used to process HNCA works well with other HNCA spectra (15N13C sample, no deuterium).
The only difference is in this sample we have deuterium decoupling. I have also attached the image of HSQC plane
with respect to 2D HSQC.

I would highly appreciate your kind help if anyone would suggest a possible way to tackle this issue.

Thanks
Vince

PS: Is there is a way to use sparkys ucsfdata to correct the mirroring.

Inline image