Dear Sparky group,

I have a 3D HNCA spectra (15N13C2H sample with deuterium decoupling) acquired on VNMRJ800.

I use the NMRpipe to convert the data into ucsf format. When I loaded ucsf file to sparky the third

dimension (Ca) looks correct, but the HSQC plane (of HNCA) shows the mirroring of the entire spectra alone
1H. I have tried to use xr xx commands to change the dimensions, found that dimensions are correct,

but all the resonances in HSQC plane flipped horizontally or 180° on 1H axis.

I dont know why.!!!

The script that I used to process HNCA works well with other HNCA spectra (15N13C sample, no deuterium).

The only difference is in this sample we have deuterium decoupling. I have also attached the image of HSQC plane

with respect to 2D HSQC.

I would highly appreciate your kind help if anyone would suggest a possible way to tackle this issue.

Thanks

Vince

PS: Is there is a way to use sparkys ucsfdata to correct the mirroring.