Subject: Re: [nmr_sparky] Re: Errors in Pre-assignment File
From: woonghee_lee
Date: Aug 8, 2017

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Hi,

Now I fully understand.
If you just compare assignments between two N-HSQCs, you can use np (Perturbation Plot).
If you want to selectively apply a few assignments from existing N-HSQC, you can do this.

1. Prepare peaks in experiments from your mutant (ae)
2. Delete all the peaks in the mtN-HSQC
2. Copy all the assignments from wtN-HSQC and paste to mtN-HSQC (pa, oc in wt and op in mt).
3. Adjust positions in mtNHSQC. Delete unmatching peaks.
4. Discard wildtype experiments in the project. And type dr to clear unused resonances.
5. Run PINE with pre-assignment.

PINE will use assignments remaining in mtN-HSQC.

Woonghee