I want togeneratePre-assignmentfile for my already known assignment for my protein using ep command through NMRFAM-SPARKY 1.41 powered by Sparky3.131.For runningthe Pre-assignmentfileat PINE Server v.2.0 orPINE Server v.3.0. However each time I am getting error message % Error: Pre-assignment file is not in the correct format. . I am attaching the peak list of myalreadyknown assignedHSQC and protein sequence file. Is error due to negative peak heights in my HSQCpeak list. Plz suggest solution.