Subject: PONDEROSA C/S (proton completeness error)
From: kiran.shivaiah
Date: Apr 1, 2016

Next: 1100


Hello Dr. Wonnghee Lee,

Thank you so much for your inputs. I finished running validation of chemical shifts using LACS. I am got only 3 amino acids with error in CA and CB values . But am not able understand how to interpret the output data (my assignments looks fine).

Secondly, when I am trying to run PONDEROSA C/S for NOESY assignment, I getting an error message which says Proton completeness is less than 60% (When I checked text message in the folder its shows29.591837%) . I assume proton completeness is number of matched observed NOEs and the number of expected NOEs, correct me if am wrong. Moreover When I add C13 edited NOESY it shows spectrum is folded we recommend it to unfold. is this could be the reason for the low proton completeness. Moreover program crashes after this showing error in python

Please let know where am I going wrong. Thank you for everything.

Best regards

Kiran .