Dear Woonghee,

So far I have finished assigning following spectrum.

1. CBCACONH

2. CBCANH

3. HNCA

4. HNCOCA

5. HNCO

6. HBHACONH

7. CCONH
8. HCCONH

Please let me know what are next few steps to calculate the structure of my protein of interest?

I am facing problem in the following sections.

1. I couldnt run LACS to verify the chemical shifts error.

2. Can you please make a video and share regarding C-HSQC assigment.

Sorry for bombarding with so many queries in one go. Please try to answer them at your convenience.

Thank you

Kiran