Subject: Re: Side chain assignment and CHSQC assignment
From: woonghee_lee
Date: Feb 23, 2016

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Dear Kiran,

The peaks generated are not random. They are from statistics but still far from reality.
You cannot expect assignments to be predicted that accurate unfortunately.

What do you have for side chains? If you have C-HSQC, C(CO)NH, H(CCO)NH, you can run APES and submit to PINE. If you dont have C(CO)NH and H(CCO)NH, it will be too difficult for you to assign side chains.

Woonghee