Hello,

I am currently using NMRFAM SPARKY for protein structure calculations. I am so far successful in backbone assignment.

I am not able to do side chain assignment on my HCCH-TOCSY and C-HSQC.(I need to also assign HNHA) It is labeling empty spaces in the spectrum where I dont see any peaks. My protein 100 a.as long. IN HCCH it shows it found 1982 peaks all of them are in empty place.

Please let me know whether we need to use command XX and XR on side chain 3D experiments too.

Thank you

Kiran Shivaiah

Graduate Student

Iowa State University