Subject: Re: [nmr_sparky] Solution structure for Protein - WorkFlow
From: Marco Sette
Date: Jan 19, 2016

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Hi Insiya,
since your protein is not too big I suggest you to use Sparky for manual assignment (manual peak picking and manual assignment) using the standard strategy. Once assigned you can use Pine to verify that you were right.
Then move to the NOESY data with your assignments and calculate the structure.

Best,
Marco

Dr.Marco Sette, Ph.D.
Department of Chemical Sciences and Technology
University of Rome, Tor Vergata
via della Ricerca Scientifica, 00133, Rome, Italy
e-mail: sette@...
e-mail: m77it@...
Tel.: +39-0672594424
Fax: +39-0672594328
http://stc.uniroma2.it/?page_id=622cn-entry-slug=marco-sette



Da: Insiya Fidai insiya.fidai@... [nmr_sparky] nmr_sparky@yahoogroups.com
A: Woonghee Lee woonghee791113@... [nmr_sparky] nmr_sparky@yahoogroups.com
Inviato: Martedì 19 Gennaio 2016 17:59
Oggetto: [nmr_sparky] Solution structure for Protein - WorkFlow

Hello,

I am working on resolving a protein structure using solution NMR. I have processed all the data using NMRPipe (HSQC, HNCO, HNCACB, CBCA(CO)NH, HCCH-TOCSY, NOESY-HSQC) and I have explored all the NMRFAM-Sparky tutorials.