Hello,
I am working on resolving a protein structure using solution NMR. I have processed all the data using NMRPipe (HSQC, HNCO, HNCACB, CBCA(CO)NH, HCCH-TOCSY, NOESY-HSQC) and I have explored all the NMRFAM-Sparky tutorials.
Firstly, the video tutorials are extremely helpful. Thank you for compiling those.
I just wanted to know what would be the best workflow to consider when resolving this structure?
Should I use APES to auto pick all the backbone and side chain peaks, then submit that to PINE for validation and for NOESY use Ponderosa or work with TALOS? There are so many cool tools to utilize, but at the same time I am on a deadline and would like to get this protein structure as soon as possible. It is a 79 residue protein.
Any advise/suggestions would be greatly appreciated.