Dear Woonghe,

thank you so much the your suggestions. In the video it is clear this is actually what I wanted know. In this method deviation is negligible and directly editing the peak list will align the side chain peaks to reference HN peak with zero deviation.

Again, thank you so much for your help and support...

Bonne week-end...

thanks

Vasantha

On Saturday, August 8, 2015 7:48 PM, Woonghee Lee woonghee791113@... [nmr_sparky] nmr_sparky@yahoogroups.com wrote:

Dear Vasantha,

I made a demonstration video.

You can watch it from here:

https://youtu.be/_Exhi3w50hg

Can you confirm if that is what you want to do?

Thanks,

Woonghee

On Sat, Aug 8, 2015 at 9:51 AM, Woonghee Lee woonghee791113@... wrote:

DearVasantha,

I can think two methods for now.

First, probably is not using peak centering or not dragging a box to pick a peak but only clicking on it in the fairly zoomed in screen.

Second, you can save peak list file, edit in text editor, read it by rp.

There may many other workarounds.

Best,

Woonghee

On Sat, Aug 8, 2015 at 5:54 AM, kumar.cerm@... [nmr_sparky] nmr_sparky@yahoogroups.com wrote:

Dear All

I have a quick question on the automatic alignment of the assigned side chain peaks belongs to the same HN peak.Automatic peak picking or peak centering on the side chains (F1) changes the peak position if spectra are not well resolved. Because of that sparky generates deviation if peaks of HA HN HG HD are not perfectly aligned to same HN frequency.

In cara/neasy there is a option if I select all side chain peaks give command move reference it aligns all the side chain peaks to same HNs..

So I am searching for the same command in SPARKY. I have to deal with natural abundance peptide only 2D tcosy/noesy spectra. Your suggestions would be a great help...

thanks
Bonne weak-end
Vasantha